LIU Wei;HE Cai;JIN Na;LI Dong;SHI Xingyun;LIU Peng;Wuwei Academy of Forestry Science;Biomass and Bioenergy Research Centre,College of Plant Science & Technology,Huazhong Agricultural University;

LIU Wei%HE Cai%JIN Na%LI Dong%SHI Xingyun%LIU Peng%Wuwei Academy of Forestry Science%Biomass and Bioenergy Research Centre,College of Plant Science & Technology,Huazhong Agricultural University

leaf blight of Lycium barbarum;;antagonistic bacteria;;antifungal activity;;control effect

In order to obtain bacillus which has strong antagonistic effect on leaf blight of Lycium barbarum,151 strains of bacillus were isolated and purified from farmland soil,and found a strain LKYLW-1 that antibacterial bandwidth was 15.2 mm with good antagonistic activity against leaf blight of L.barbarum.The antibacterial effect of LKYLW-1 was measured by pair culture,spore germination and the field efficacy trials.The results showed that the strain LKYLW-1 had teratogenic effects on the mycelium of the leaf blight of L.barbarum.The fermentation broth of strain LKYLW-1 could inhibit the spore germination,and the EC_(50) was 9.35 mL·L~(-1).In the field efficacy trials,the antagonistic strain LKYLW-1 showed good preventive and therapeutic effects on leaf blight of L.barbarum.The strain LKYLW-1 was identified as Bacillus atrophaeus by morphological characteristics,physical and chemical analysis and 16 S rDNA sequence analysis,which could produce cellulase,protease and amylase during metabolism.So the strain LKYLW-1 has potential utilization value on the control of leaf blight of L.barbarum.