中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 猪印记基因MKRN3的SNPs筛选及胴体性状关联分析 华中农业大学学报 2017,36 (6) 77-82
Position: Home > Articles > SNPs identification of porcine imprinting gene MKRN3 and association analysis with carcass traits Journal of Huazhong Agricultural University 2017,36 (6) 77-82

猪印记基因MKRN3的SNPs筛选及胴体性状关联分析

作  者：
汪龙梅;郭玲;刘玉兰;付书林;陈洪波;张晶;邓昌彦
单  位：
农业动物遗传育种与繁殖教育部重点实验室;武汉轻工大学动物营养与饲料科学湖北省重点实验室
关键词：
猪;MKRN3;SNPs;内脂率;臀部膘厚;印记基因
摘  要：
以梅山猪、大白猪和长白猪为研究对象,筛选出MRKN3基因的单核苷酸多态位点(SNPs)并进行PCR-RFLP验证,利用PCR-Bst UI-RFLP对大白×梅山F2代资源家系(285头)DNA样品酶切分型,将分型结果与猪胴体性状进行关联分析。结果表明:猪MRKN3基因编码区有7个SNPs,分别为293C>G、361G>A、497G>A、630G>A、678T>C、1376C>T、和1407T>A;其中678T>C位点等位基因频率在中外不同猪种中存在差异;猪MRKN3基因678T>C位点与内脂率及臀部膘厚显著相关(P<0.05);678T>C位点对内脂率具有显性效应(P<0.05),表现为该位点杂合子比纯合子具有更高的内脂率;而该位点对臀部膘厚具有加性效应(P<0.05),表现为等位基因678C的累加具有降低臀部膘厚的效应。因此,猪MKRN3基因的678T>C位点可为猪的分子育种实践提供一定指导作用。
译  名：
SNPs identification of porcine imprinting gene MKRN3 and association analysis with carcass traits
作  者：
WANG Longmei;GUO Ling;LIU Yulan;FU Shulin;CHEN Hongbo;ZHANG Jing;DENG Changyan;Hubei Key Laboratory of Animal Nutrition and Feed Science,Wuhan Polytechnic University;Key Laboratory of Agricultural Animal Genetics,Breeding and Reproduction of Ministry of Education,Huazhong Agricultural University;
单  位：
Hubei Key Laboratory of Animal Nutrition and Feed Science,Wuhan Polytechnic University%Key Laboratory of Agricultural Animal Genetics,Breeding and Reproduction of Ministry of Education,Huazhong Agricultural University
关键词：
pig;;MKRN3;;SNPs;;internal fat rate;;buttock fat thickness;;imprinting gene
摘  要：
Single nucleotide polymorphisms(SNPs)of the porcine imprinting gene MKRN3 were identified in Meishan,Large white and Landrace pigs by PCR-RFLP and were genotyped in the F2 population of 285 Large white × Meishan pigs by PCR-Bst UI-RFLP.Subsequently,correlation between the porcine gene MKRN3 polymorphism and porcine carcass traits were analyzed.The results showed that,the porcine MKRN3 gene had seven SNPs in the coding region,including 293 C>G,361 G>A,497 G>A,630 G>A,678 T>C,1376 C>T,and 1407 T>A.Of which,the 678 T>C site had different allele frequencies among domestic and foreigner pig species.In addition,the 678 T>C site of the porcine MKRN3 was associated with internal fat rate and buttock fat thickness significantly(P<0.05).The 678 T>C site had a dominant effect on internal fat rate(P<0.05),showing an increased internal fat rate with heterozygote form and had an additive effect on buttock fat thickness(P <0.05),showing a decreased buttock fat thickness with the additive of the 678 Callele.Therefore,the 678 T>C site of the porcine MKRN3 might provide a guiding value for the molecular breeding practice.

相似文章

导出引用
计量
文章访问数: 8
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46