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当前位置: 首页 > 文章 > ‘泰山早霞’苹果采后1-甲基环丙烯处理对其软化及相关基因表达的影响 园艺学报 2012,39 (5) 845-852
Position: Home > Articles > The Regulation of 1-methylcyclopropene on Softening and Expression of Relevant Genes in‘Taishan Zaoxia’Apple Acta Horticulturae Sinica 2012,39 (5) 845-852

‘泰山早霞’苹果采后1-甲基环丙烯处理对其软化及相关基因表达的影响

作  者：
刘美艳;魏景利;刘金;房龙;宋杨
单  位：
西南交通大学土木工程学院;山东省冠县国有毛白杨林场;山东农业大学作物生物学国家重点实验室
关键词：
苹果;早熟;贮藏;软化;1-MCP;细胞壁酶;基因;表达
摘  要：
以‘泰山早霞’苹果成熟的果实为试材,于采收当天进行1–甲基环丙烯(1-MCP)处理,研究其对果实软化及相关基因表达的影响。结果表明,①1-MCP处理的果实在短期贮藏期间硬度均明显高于对照。②1-MCP处理后,乙烯释放速率、PG、PME、β-Gal、α-L-Af及LOX等细胞壁酶基因的表达均被明显抑制,在整个试验期内均有明显下降,尤其是在处理后1d,就分别比各自的对照下降了72.0%、72.1%、87.5%、81.8%、90.2%和16.7%,而1-MCP对AM和XET基因的表达没有明显作用。上述结果表明,苹果‘泰山早霞’品种属乙烯极敏感型,1-MCP对于延缓其果实软化具有明显作用,其果实软化可能是PG、PME、β-Gal、α-L-Af及LOX等多种基因协同作用的结果。
译  名：
The Regulation of 1-methylcyclopropene on Softening and Expression of Relevant Genes in‘Taishan Zaoxia’Apple
作  者：
LIU Mei-yan1,WEI Jing-li2,LIU Jin1,FANG Long1,SONG Yang1,CUI Mei1,WANG Chuan-zeng1, and CHEN Xue-sen1,* (1State Key Laboratory of Crop Biology,Shandong Agricultural Uiversity,Tai’an,Shandong 271018,China;2Guanxian State Forest Farm of Populas Tomentosa,Guanxian,Shandong 252500,China)
关键词：
apple;early-maturing;storage;softening;1-MCP;cell wall enzyme;gene;express
摘  要：
In order to study the molecular softening mechanism of‘Taishan Zaoxia’apple,the effect of 1-MCP on softening and expressions of genes related to softening in‘Taishan Zaoxia’were investigated. The results showed that:①The firmness of‘Taishan Zaoxia’fruit treated with 1-MCP were higher than the control fruit during the storage after harvest.②Ethylene production rate and the expression of PG,PME, β-Gal,α-L-Af and LOX genes of‘Taishan Zaoxia’fruit were inhibited significantly throughout the whole experimental period after treated with 1-MCP,especially in the first day after treatment. They decreased 72.0%,72.1%,87.5%,81.8%,90.2% and 16.7% than control respectively. But 1-MCP had no effect on expression of AM and XET genes. These results indicated that‘Taishan Zaoxia’apple variety was very sensitive to ethylene and 1-MCP could delay softening of‘Taishan Zaoxia’fruit obviously;The fruitsoftening may be caused by synergistic effect of PG,PME,β-Gal,α-L-Af and LOX genes.

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