Position: Home > Articles > Detection and homology analysis of CRISPRs in Lactobacillus delbrueckii
Journal of Northeast Agricultural University
2011,42
(11)
25-29
CRISPRs在德氏乳杆菌中的检测与同源性分析
作 者：
邓凯波;霍贵成;柳国霞
单 位：
乳品科学教育部重点实验室
关键词：
CRISPRs;德氏乳杆菌;检测;同源性
摘 要：
本研究检测了本实验室德氏乳杆菌中的CRISPRs结构,并对其重复序列和间区序列进行了同源性分析。结果显示,供试菌株L7和L9中均含有CRISPRs序列,分别为3 323 bp和3 015 bp,多重比对后的重复序列8～12位与21～17位碱基互补形成回文结构;重复序列大小(29 bp)与标准菌株基本相同,但其同源性与菌株亲缘关系具差异性,验证了CRISPRs结构的水平基因转移的特点。
译 名：
Detection and homology analysis of CRISPRs in Lactobacillus delbrueckii
作 者：
DENG Kaibo1,2,HUO Guicheng1,2,LIU Guoxia1,2(1.Key Laboratory of Dairy Science,Ministry of Education,Harbin 150030,China;2.Food College,Northeast Agricultural University,Harbin 150030,China)
关键词：
CRISPRs;Lactobacillus delbrueckii;detection;homology
摘 要：
This study detected CRISPRs of Lactobacillus delbrueckii in KLDS,and analyzed the homology of their direct repeats and spacer sequences.The results showed that L7 and L9 had CRISPRs which were 3 323 bp and 3 015 bp respectively.The consensus direct repeat after multiple alignments formatted a palindromic structure as base-pairing between 8-12 and 21-17 bases;the direct repeats had the same size(29 bp) as the typical strains,but the homology possessed variability compared with the genetic relationship,which validated the characters of CRISPRs horizontal gene transfer.
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