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Position: Home > Articles > Isolation and Characterization of an Avian Encephalon Escherichia coli Strain Chinese Journal of Veterinary Science 2000,20 (1) 27-30

1株鸡源神经型大肠埃希氏菌的分离及特性分析

作  者:
恽时锋;潘震寰
单  位:
南京军区南京总医院动物实验科
关键词:
神经型;大肠埃希氏菌;血清型;菌毛;血凝性;全菌蛋白;致病性
摘  要:
从某鸡场以瘫痪、歪颈、昏睡为主要症状的肉雏鸡脑实质中 ,分离到 1株革兰氏阴性、有动力的短杆菌 ,定名为 LCB。其主要生化特性为氧化酶阴性、接触酶阳性 ,发酵葡萄糖、甘露醇 ,产酸产气 ,发酵乳糖、山梨醇 ,不发酵蔗糖、卫茅醇、肌醇 ,不产生硫化氢 ,不液化明胶 ,不分解尿素 ,M- R阳性 ,V - P阴性。经鉴定为大肠埃希氏菌 ,血清型属 O131,为国内外新发现的鸡大肠埃希氏菌的血清型。该菌株腹腔接种对 K M小鼠具有高致病性 ,脑内接种对 1日龄雏鸡也具高致病性 ,但对鸡腹腔及气管接种则无致病性。在 N B中经 37℃ 72 h培养不表达菌毛。能凝集鸭、犬、鸡、豚鼠、马的 RBC,并能被 D -甘露糖抑制 ,表现为甘露糖敏感性血凝。全菌蛋白 SDS- PAGE电泳及免疫转印表明 ,该菌株缺失鸡致病性大肠埃希氏菌 MG4 0所特有的大小约为4 0 0 0 0的 1条多肽。
译  名:
Isolation and Characterization of an Avian Encephalon Escherichia coli Strain
作  者:
YUN Shi feng, PAN Zhen huan (Department of Animal Experiment, Nanjing General Hospital in Nanjing Military Area, Nanjing 210002, China)[KH8/9D]
关键词:
encephalopathy; Escherichia coli; serogroup; fimbriae; hemagglutination; whole bacterial protein; pathogenicity
摘  要:
The Escherichia coli called as LCB was isolated from an encephalon material of a dead broiler manifested the symptom of paralysis, wry neck and lethargy in Lianyungang areas of Jiangsu province. The biochemical and physical characteristics of the strain were similar to those of the avian E. coli MG40 and Q50. The serotype of LCB belong to O113, a newly discovered on avian E.coli serogroup. Pathogenicity of LCB to KM mice was high through intraperitoneal injection, as well as to one day old chicks through intracranial injection. Nonpathogenicity was shown to chicks through intraperitoneal and intratracheal inoculation. No fimbriae of LCB was observed through 37℃ culture for 72 h in NB, but exhibited the mannose sensitive and hemagglutination (MSHA) of erythyrocytes of duck, canine, chicken, guinea pig, horse. By SDS PAGE of whole bacterial proteins, LCB was proved to be deleted of one major protein subunits of 40 000 which exists only in pathogenic strains MG40.

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