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当前位置: 首页 > 文章 > 番木瓜抗环斑病毒突变体抗性遗传及RAPD标记 植物病理学报 2001,31 (2) 157-163
Position: Home > Articles > HEREDITY AND RAPD MARKER OF PAPAYA MUTANT RESISTANCE TO PRSV Acta Phytopathologica Sinica 2001,31 (2) 157-163

番木瓜抗环斑病毒突变体抗性遗传及RAPD标记

作  者：
周国辉;李华平;张曙光;饶雪琴;王振中;范怀忠
单  位：
华南农业大学植物病毒研究室
关键词：
番木瓜环斑病毒;抗病突变体;RAPD分子标记
摘  要：
:60 Coγ-射线处理番木瓜种子 ,从诱变一代中筛选到 1株耐环斑病毒 ( PRSV)的变异植株 ( M1) ,其侧芽组培后代 ( VM1)部分植株也表现出了耐病性 ,以 VM1为母本进行回交 ,人工接种病毒鉴定回交后代 ( BM2 )的抗病性 ,结果为 :在出自部分回交果实的 BM2 群体中 ,包含有对 PRSV Ys和 Vb株系具抗性的植株 ,抗感分离比为 1∶ 1,但未发现抗 Sm株系的植株 ;BM2 抗病两性株自交或以其为母本进行回交 ,分别获得诱变第三代 ( M3)及回交诱变第三代 ( BM3) ,人工接种 PRSV Ys株系 ,结果表明 ,BM3抗感分离比也为 1∶ 1,因而认为辐射处理突变产生了具 PRSV株系专化性的显性抗病基因 ,命名为 Rys;但 M3抗感分离比不符合显性单基因 3∶ 1理论值 ,认为是单倍体选择的结果。运用 BSA法 ,在 BM2 中寻找到一个与抗病性密切相关的 RAPD标记 ,经在 BM2 、M3及 BM3抗感群体中检验 ,可作为抗病育种的辅助选择标记。Rys是番木瓜栽培种中发现的第一个抗 PRSV基因
译  名：
HEREDITY AND RAPD MARKER OF PAPAYA MUTANT RESISTANCE TO PRSV
作  者：
ZHOU Guo hui, LI Hua ping, ZHANG Shu guang, LAO Xie qin, WANG Zheng zhong, FAN Huai zhong [WT6BZ](Plant Virus Laboratory, South China Agric. Univ., Guangzhou 510642, China)[HJ12] [WT5HZ][HJ2/5]
关键词：
papaya ringspot potyvirus; resistant mutant; RAPD marker
摘  要：
A papaya ( Carica papaya L.) mutant plant (M 1) with tolerance to papaya ringspot potyvirus (PRSV) was selected from the first mutated generation population after 60 Co γ ray irradiating seeds. A part of plants (VM 1) propagated vegetatively from the lateral shoots of M 1 plant still showed tolerance to PRSV. Back cross was performed using VM 1 as maternal parent and the seedlings (BM 2) were developed from the seeds of individual fruit. The resistance of BM 2 plants was evaluated by artificial inoculation with PRSV Ys, Vb and Sm strains. The BM 2 populations showed that their resistance and susceptibility to PRSV Ys and Vb strains were in the ratio of one to one. But none was found to be resistant to Sm strain in tested BM 2 populations. The segregation ratio in BM 3 population derived from the back cross of BM 2 resistant plants as maternal parent fit the theoretical value (1∶1). The results suggested that γ ray irradiation might has induced a single dominant gene resisting to PRSV Ys and Vb, named Rys. Because the segregation ratio of resistant and susceptible plants to PRSV Ys in M 3 population derived from BM 2 resistant plant self pollination did not fit the theoretical value (3∶1) of single dominant gene character, a part of male gametes with mutated gene were thought to be excluded during their development and fertilization. Using bulked segregant analysis (BSA), a RAPD marker significantly related with the resistance was identified and will be of great use for aiding resistance screening in papaya breeding. Rys is the first PRSV resistant gene found in papaya cultivar.

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