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当前位置: 首页 > 文章 > 番茄颈腐根腐病病原菌鉴定与抗病种质材料的筛选 园艺学报 2016,43 (4) 781-788
Position: Home > Articles > Pathogen Identification of Fusarium Crown Root Rot and Screening for Resistant Sources in Tomato Acta Horticulturae Sinica 2016,43 (4) 781-788

番茄颈腐根腐病病原菌鉴定与抗病种质材料的筛选

作  者：
程琳;张生;李艳青;陈福东;程斐;张晓艳;董甜;国家进
单  位：
青岛农业大学;潍坊科技学院;山东省设施蔬菜分子育种省级重点实验室
关键词：
番茄;番茄颈腐根腐病;病原菌鉴定;Frl;分子标记
摘  要：
2014年在山东寿光农户种植番茄的日光温室内采集典型颈腐根腐病症状的发病植株进行病原分离、纯化,结合形态学观察及rDNA-ITS序列分析,确定病原菌为尖孢镰刀菌番茄颈腐根腐病专化型(Fusarium oxysporum f.sp.radicis-lycopersici)。利用源自秘鲁番茄颈腐根腐病抗性基因Frl的共显性标记C2-25,对田间未表现典型颈腐根腐病症状的25份种质材料进行鉴定,鉴定出19份纯合抗病材料,2份杂合抗病材料,4份纯合感病材料,此结果与人工接种病原菌鉴定方法相一致。
译  名：
Pathogen Identification of Fusarium Crown Root Rot and Screening for Resistant Sources in Tomato
作  者：
CHENG Lin;ZHANG Sheng;LI Yan-qing;CHEN Fu-dong;CHENG Fei;ZHANG Xiao-yan;DONG Tian;GUO Jia-jin;Shandong Provincial Key Laboratory of Protected Vegetable Molecular Breeding,Weifang Enterprise Key Laboratory of Vegetable Molecular Breeding,Shandong Shouguang Vegetable Seed Industry Group Co.Ltd,Shandong Shouguang Vegetable Industry Group Co.Ltd;Weifang University of Science & Technology;Qingdao Agricultural University;
关键词：
tomato;;Fusarium crown and root rot;;pathogen identification;;Frl;;molecular marker
摘  要：
The typical Fusarium crown root rot(FCRR)infected tomato plants were collected in growers' greenhouse in Shouguang,Shandong Province in 2014.The pathogen was isolated and catagaried according to the morphology and the sequence of internal transcribed spacer region of the ribosomal DNA(ITS).The result showed that the pathogen of the disease was identified as Fusarium oxysporum f.sp.radicis-lycopersici.The cleaved amplified polymorphic sequence(CAPS)marker C2-25 for FCRR resistance gene Frl,which was derived from Solanum peruvianum,were tested in 25 germplasms without typical FCRR symptom,in which 19 homozygous,2 heterozygous resistants and 4 homozygous susceptible plants were found.Moreover,molecular marker test showed good agreement with the artificial pathogen inoculation results.

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