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当前位置: 首页 > 文章 > 欧美杨107杨β-1,3-葡聚糖酶(BG2)基因遗传转化及对溃疡病的抗性分析 林业科学 2013,49 (11) 60-66
Position: Home > Articles > Transformation of BG 2 Gene into Populus × euramericana cv. ‘Neva'and Resistance of the Transgenic Poplar to Canker Disease Scientia Silvae Sinicae 2013,49 (11) 60-66

欧美杨107杨β-1,3-葡聚糖酶(BG2)基因遗传转化及对溃疡病的抗性分析

作  者：
牛庆霖;王迎;罗磊;黄艳艳;刘静;冯殿齐;曹帮华
单  位：
山东农业大学农业生态与环境重点实验室;泰安市泰山林业科学研究院
关键词：
欧美杨107杨;根癌农杆菌;BG2基因;基因转化;溃疡病
摘  要：
以欧美杨107杨为受体材料,利用根癌农杆菌介导法转化β-1,3-葡聚糖酶基因(BG2),经卡那霉素筛选,PCR和Southern检测显示有4个转基因阳性株系,初步证明外源目的基因已整合到107杨基因组中。离体抑菌试验表明转基因107杨(T-107杨)细胞粗提液的抑菌能力明显强于未转基因对照107杨(C-107杨)。在接种杨树溃疡病菌的培养基上培养,T-107杨长势明显强于C-107杨;T-107杨可明显抑制丙二醛(MDA)在植物体内的快速积累,其苯丙氨酸解氨酶(PAL)含量也明显高于C-107杨,PAL含量T-107杨和C-107杨都有先升后降的趋势;15天后C-107杨完全死亡,T-107杨仍能生存。试验结果表明BG2基因的表达提高了107杨对杨树溃疡病的抗性。
译  名：
Transformation of BG 2 Gene into Populus × euramericana cv. ‘Neva'and Resistance of the Transgenic Poplar to Canker Disease
作  者：
Niu Qinglin;Wang Ying;Luo Lei;Huang Yanyan;Liu Jing;Feng Dianqi;Cao Banghua;Key Laboratory of Agricultural Ecology and Environment,Shandong Agricultural University;Taishan Institute of Forestry Science;
关键词：
Populus × euramericana cv.‘Neva ';;Agrobacterium tumefaciens;;BG 2 gene;;gene transformation;;canker disease
摘  要：
The BG 2 gene was transformed into Populus × euramericana cv. ‘Neva ' via Agrobacterium tumefaciens mediated transformation method. Four transgenic lines were obtained through selection of kanamycin resistance,PCR and Southern analyses. These analyses suggested that the BG 2 gene was integrated into the plant genome. Then,the transgenic plants were tested for resistance to the fungal pathogen Cytospora chrysosperma. Crude extracts of the transgenic T-107 plants exhibited in vitro inhibitory activity against the fungal pathogen. When inoculated with Populus × euramericana canker disease,the T-107 plants grew better than the C-107 plants. The MDA levels of T-107 were lower than that in C-107. The PAL contents of both transgenic and control plants were firstly increased obviously then decreased; However, the PAL content of T-107 plants was always higher than that in C-107 plants. After 15 days,all the C-107 plants dead, but the T-107 was still alive. These results indicated that the transformed BG 2 gene could improve the resistance of T-107 plants to canker disease.

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