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当前位置: 首页 > 文章 > 应用花培技术培育水稻籼粳交两系不育材料 南方农业学报 2013,44 (11) 1771-1775
Position: Home > Articles > Breeding stable two-line sterile material of indica-japonica hybrid using anther culture Journal of Southern Agriculture 2013,44 (11) 1771-1775

应用花培技术培育水稻籼粳交两系不育材料

作  者：
姚国新;黄文超;但志武
单  位：
武汉大学
关键词：
杂种优势;籼粳交;两系不育系;花培技术
摘  要：
【目的】快速将粳稻染色体片段渗入籼型两系不育系,获得稳定材料,便于亚种间杂种优势利用。【方法】对籼型两系不育系Bph68S与粳稻少孽粳杂交的F1,采用花药培养的方法,构建籼粳交DH系两系材料,并观察其杂种优势表现。【结果】对大田的种植株型进行鉴定,从242株绿苗中获得123个二倍体单株,其中41个单株具有两系不育系的特性;DH3S两系不育单株具有较强的杂种优势,与母本Bph68S相比,其有效穗降低(14.2±2.9)%,成穗率增加(21.5±5.8)%,株高降低(28.5±3.2)%。两系不育株DH3S与籼稻9311组配杂交种F1的成穗率、穗粒数和单株产量均高于Bph68S与9311组配的杂交组合。【结论】用籼稻两系不育亲本与粳稻杂交的F1花培构建DH两系不育材料,可快速获得两系不育系稳定中间材料,是快速获得籼粳交不育系的可行方法。
译  名：
Breeding stable two-line sterile material of indica-japonica hybrid using anther culture
作  者：
YAO Guo-xin;HUANG Wen-chao;DAN Zhi-wu;College of Life Science,Wuhan University;School of Life and Science Technology,Hubei Engineering University;
关键词：
heterosis;;indica-japonica hybrid;;two-line;;anther culture
摘  要：
【Objective】Rapid introgression fragments of japonica to two-line sterile was conducted to obtain stable material could facilitate application of heterosis of subspecific hybrid.【Method】In this study,cross F1 between two-line indica Bph68S and japonica SNJ,was used for constructing two-line sterile material of indica-japonica hybrid using anther culture method to record the performance of superior hybrid in observation time.【Result】After identification of the plants cultured in field,123 diploid plants of indica and japonica hybrid were got out of 242 plants,which 41 plants showed characteristics of two-line traits sterile.DH3S two-line sterile plants,compared to its female parent Bph68S,its effective panicle was reduced by(14.2±2.9)%,mature panicle ratio decreased(21.5±5.8)%,and plant height was down by(28.5± 3.2)%.The spike rate,spike grains and grain yield per plant of the F1 of DH3s and 9311 were all higher than those of the combination between Bph68S and 9311.【 Conclusion 】 Through anther culture of cross F1 between twoline sterile indica Bph68S and japonica SNJ to construct DH two-line sterile material is feasible for rapidly breeding new sterile line of indica-japonica hybrid.

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