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当前位置: 首页 > 文章 > GmSbh1在大豆种子发育、响应高温高湿和激素诱导中的表达分析 分子植物育种 2016,14 (10) 2560-2566
Position: Home > Articles > Expression Analysis of GmSbh1 Involved in Seed Development and Response to High Temperature and Humidity Stress and Hormone Induction in Soybean(Glycine max(L.) Merr.) Molecular Plant Breeding 2016,14 (10) 2560-2566

GmSbh1在大豆种子发育、响应高温高湿和激素诱导中的表达分析

作  者：
舒英杰;周玉丽;陶源;胡能兵;黄守程
单  位：
安徽科技学院植物科学学院;安徽科技学院;安徽科技学院生命科学学院
关键词：
大豆;GmSbh1基因;高温高湿;激素诱导;特异性表达
摘  要：
GmSbh1(L13663)是大豆中被克隆的第一个同源异型盒(homeobox,HB)基因。该类基因在调控植物生长发育、响应逆境胁迫和激素诱导等方面发挥重要作用。本研究采用q RT-PCR技术,分析GmSbh1基因在大豆不同组织器官、叶片与种子发育、种子萌发以及响应高温高湿胁迫和激素诱导中的表达模式。结果表明,GmSbh1基因在大豆种子中的表达量高于幼苗,子叶和胚中的表达高于种皮,幼苗下胚轴、真叶和根中的表达量很低。大豆种子发育成熟过程中GmSbh1表达量逐渐上升,生理成熟期(R7期)表达量显著增加,完熟期(R8期)表达量最高;种子萌发过程中GmSbh1表达量呈先升后降趋势,吸胀24~48 h表达量上升,随后下降;随着大豆叶片的发育和衰老,GmSbh1表达呈先升后降趋势,功能叶期表达量最高。高温高湿胁迫后大豆种子中的GmSbh1表达量上升,胁迫48 h后上升的幅度显著增大;与对照相比,不抗田间劣变品种宁镇1号显著上调表达,抗性品种湘豆3号下调表达。100μmol/L ABA、100μmol/L Me JA、100μmol/L GA3和20μmol/L IAA处理宁镇1号1 h后,GmSbh1的表达量显著升高,随着处里时间的延长,表达量降低;同样浓度的几种激素处里湘豆3号后,GmSbh1表达变化的规律不明显。以上结果表明,GmSbh1基因可能参与大豆种子与叶片的发育,响应高温高湿胁迫和激素诱导。
译  名：
Expression Analysis of GmSbh1 Involved in Seed Development and Response to High Temperature and Humidity Stress and Hormone Induction in Soybean(Glycine max(L.) Merr.)
作  者：
Shu Yingjie;Zhou Yuli;Tao Yuan;Hu Nengbing;Huang Shoucheng;He Qingyuan;Ma Hao;University of Science and Technology of Anhui;State Key Laboratory of Crop Genetics and Germplasm Enhancement,Nanjing Agricultural University;
关键词：
Soybean(Glycine max(L.) Merr.);;GmSbh1;;High temperature and humidity;;Hormone induction;;Specific expression
摘  要：
Homeobox genes are playing an important role in regulating plant growth and development and responding to biotic and abiotic stresses or hormone induction. GmSbh1(L13663) is the first homeobox gene that cloned from soybean. Real-time quantitative PCR analysis showed that GmSbh1 was expressed in seed and seedlings of soybean, but the expression level of seed was higher than seedling. We also found that, the expression level of GmSbh1 incotyledon and embryo were higher than the seed coat, and the expression levels of GmSbh1 in hypocotyl, euphylla and root were weak. The expression of GmSbh1 increased with the development and maturation of soybean seed, and there was the highest expression level at full ripe period(R8period). GmSbh1 upregulated expression when seed imbibition from 24 hours to 48 hours, then down-regulated expression. With the leaf development and senescence, the expression level showed a rise first followed by a decline, and functional leaves have a highest expression level. T he expression increased with high temperature and humidity stress time extended, especially when the stress time after 48 hours. We can found that up-regulated expression compared with control in Ningzhen No.1, and down-regulated expression in Xiangdou No.3 under stress condition at the physiological maturation stage. After one hour treatment with 100 μmol/L ABA, 100 μmol/L Me JA, 100 μmol/L GA3 and 20 μmol/L IAA, respectively, the transcriptionnal expression of GmSbh1 in leaves significantly increased and decline gradually in Ningzhen No.1. The GmSbh1 expression had no regular change in Xiangdou No.3 when it was treated with the same hormone used in Ningzhen No.1. This results showed that GmSbh1 may involved in seed development and response to high temperature and humidity stress and hormone induction in soybean.

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