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当前位置: 首页 > 文章 > 北京鸭RIG-I基因的克隆及其生物信息学分析 浙江农业学报 2015,27 (5) 734-739
Position: Home > Articles > Cloning and bioinformatics analysis of Peking Duck Retinoic Acid Inducible Gene I Acta Agriculturae Zhejiangensis 2015,27 (5) 734-739

北京鸭RIG-I基因的克隆及其生物信息学分析

作  者：
王同淑;李传峰;陈宗艳;孟春春;吴润
单  位：
甘肃农业大学动物医学院;中国农业科学院上海兽医研究所;甘肃农业大学动物医学院中国农业科学院上海兽医研究所
关键词：
鸭;RIG-I;克隆;生物信息学分析;进化树
摘  要：
应用RT-PCR方法,从北京鸭组织中扩增维甲酸诱导基因I(rerinotic acid inducible gene I,RIG-I),用DNA Star等生物软件分析其所含的生物信息,并构建系统进化树。扩增出的北京鸭RIG-I基因(Gen Bank登录号:KM455977)全长2 802 bp,编码933个氨基酸。RIG-I具有RLR家族的典型特征,即N端含两个半胱天冬酶活化和募集结构域,C端包括RNA解旋酶,ATP结合结构域,RNA结合结构域和抑制结构域,RIG-I蛋白二级结构以无规则卷曲和α-螺旋为主。序列比对和系统进化分析结果表明,RIG-I的序列在同种之间比较保守,与已报道的鸭RIG-I的同源性高达98%以上;但与鹅RIG-I同源性稍低,为95.9%;与人、鼠、草鱼的同源性较低,分别为62.3%,61.5%,61.2%;与猪的同源性最低,仅为54.4%。成功克隆了鸭的RIG-I基因,可为今后开展鸭的天然免疫研究及研发新型疫苗等奠定基础。
译  名：
Cloning and bioinformatics analysis of Peking Duck Retinoic Acid Inducible Gene I
作  者：
WANG Tong-shu;LI Chuan-feng;CHEN Zong-yan;MENG Chun-chun;WU Run;LIU Guang-qin;College of Veterinary Medicine,Gansu Agricultural University;Shanghai Veterinary Research Institute,Chinese Academy of Agricultural Sciences;
关键词：
duck;;RIG-I;;cloning;;bioinformatics analysis;;phylogenetic tree
摘  要：
Duck erinotic acid inducible gene I( RIG-I) encoding sequence was cloned from spleen of Peking duck,its nucleotide sequence analysis and phylogenetic tree were performed by DNA Star. Nucleotide sequence analysis revealed a sequence of 2 802 bp in length,RIG-I encoded a predicted polypeptide of 933 amino acids. RIG-I had some typical characteristics of RLR family,for example,there were two caspase activation sites and a recruit domain structure within the N-terminal of RIG-I; In the C-terminal of RIG-I,the regions of RNA helicase,ATP binding site,RNA binding site and repression domain were also present. The secondary stucture of RIG-I showed mainly random coils and alpha helix. Results of phylogenetic tree showed that the sequence of duck RIG-I was highly conserved among the same population,which shared 99% homology with the published duck RIG-I sequences. But the homology between duck and other species was lower,for example,the identities between duck and goose,homo,mouse,Ctenopharyngodon idella and susscrofa were 95. 9%,62. 3%,61. 5%,61. 2% and 54. 4%,respectively. In conclusion,duck RIG-I gene was successful cloned in this study,which would be helpful for the future study of the in-nate immune of duck and developing new type vaccines.

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