Position: Home > Articles > Distribution of 5-HT and DA in the ovary and hepatopancreas during the ovarian development in Procambrus clarkii
Freshwater Fisheries
2012,42
(6)
21-26
克氏原螯虾不同发育时期5-HT与DA在卵巢和肝胰腺中的分布
作 者：
魏华;沙晓姣;陈阿琴;唐建清;夏爱军
单 位：
上海海洋大学水产与生命科学学院;江苏省淡水水产研究所
关键词：
克氏原螯虾(Procambrus clarkii);5-HT;DA;卵巢;肝胰腺
摘 要：
为了研究5-HT与DA阳性物质在克氏原螯虾(Procambrus clarkii)不同发育时期的卵巢细胞和肝胰腺细胞中的分布特征,运用免疫组化法对卵巢发育4个不同时期的卵巢与肝胰腺进行观察。结果表明,各期卵巢卵细胞和肝胰腺细胞中均存在5-HT与DA阳性物质。Ⅰ期卵巢5-HT阳性物质分布于卵母细胞胞核,胞质未出现阳性;Ⅱ期～Ⅳ期5-HT阳性物质主要集中在卵母细胞胞质以及胞核。在发育各期的肝胰腺中5-HT的免疫物质均呈阳性。Ⅰ～Ⅳ期卵巢中,DA阳性物质主要分布于卵母细胞的胞核以及肝胰腺细胞,其强度呈递减趋势。结果提示5-HT具有促进卵巢发育作用,而DA则抑制卵巢发育。
译 名：
Distribution of 5-HT and DA in the ovary and hepatopancreas during the ovarian development in Procambrus clarkii
作 者：
WEI Hua1,2,SHA Xiao-jiao1,CHEN A-qin1,TANG Jian-qing3,XIA Ai-jun3(1.College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306; 2.Shanghai Vocational and Technical College of Agriculture and Forestry,Shanghai 201600; 3.Jiangsu Freshwater Fisheries Research Institute,Nanjing 210017)
关键词：
Procambrus clarkii;5-HT;DA;ovary;the hepatopancreas
摘 要：
The distribution of 5-HT and DA in the ovary and hepatopancreas of Procambrus clarkii was investigated in immunohistochemical method at 4 different stages of ovary development.The results showed that 5-HT and DA-positive cells were observed in ovary and hepatopancreas during the ovary development.However,the 5-HT-positive cells were only observed in the nucleus at the ovarian developmental stage I and these were observed both in the cytoplasm and nucleus at the ovarian developmental stage fromⅡto IV.The 5-HT-positive hepatopancreas cells were observed at every ovarian developmental stage.During each ovary stage,DA-positive cells were observed both in nucleus of oocyte and the hepatopancreas and DA intensity decreased with the oocyte development.The results suggested that 5-HT promoted ovarian growth while DA inhibited ovarian development in P.clarkii.
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