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当前位置: 首页 > 文章 > PEG胁迫下偏关苜蓿ProDH基因表达与活性分析 山西农业大学学报(自然科学版) 2018 (3) 52-56
Position: Home > Articles > Gene expression level and activity of ProDH in Medicago Sativa cv. Pianguan under PEG stress Journal of Shanxi Agricultural University(Natural Science Edition) 2018 (3) 52-56

PEG胁迫下偏关苜蓿ProDH基因表达与活性分析

作  者：
贾蓉;庞妙甜;原慧;熊乙;董宽虎;朱慧森
单  位：
山西农业大学动物科技学院
关键词：
偏关苜蓿;PEG胁迫;ProDH基因
摘  要：
[目的]探索偏关苜蓿在PEG模拟干旱胁迫下,脯氨酸脱氢酶(Proline Dehydrogenase,ProDH)基因表达水平及其与脯氨酸含量、脯氨酸脱氢酶活性的关系。[方法]采用20%PEG模拟干旱胁迫处理生长四周的偏关苜蓿植株,分别胁迫0h、24h、48h和72h后,测定脯氨酸含量、脯氨酸脱氢酶活性,采用qRT-PCR法检测ProDH基因的相对表达水平。[结果]随着PEG干旱胁迫时间的延长,脯氨酸的含量逐渐增加,ProDH的活性逐渐降低,ProDH基因的表达量亦表现为增加。[结论]脯氨酸脱氢酶基因表达水平与植株ProDH活性的变化不一致,表明该基因的酶活性受到了转录和翻译水平的双重调控。
译  名：
Gene expression level and activity of ProDH in Medicago Sativa cv. Pianguan under PEG stress
作  者：
Jia Rong;Pang Miaotian;Yuan Hui;Xiong Yi;Dong Kuanhu;Zhu Huisen;College of Animal science and Veterinary Medicine,Shanxi Agricultural University;
关键词：
Medicago Sativa cv. Pianguan;;PEG stress;;ProDH gene
摘  要：
[Objective]The aim of present study was to explore the relationship between gene expression levels,activity of ProDH,and proline contents in Medicago Sativa cv.Pianguan under PEG stress.[Methods]The alfalfa were treated with PEG(20%)for 0 h,24 h,48 hand 72 h,and the proline contents,activities and expression of ProDH mRNA of the plants were determined using qRT-PCR.[Results]The proline contents and expression of ProDH were increased constantly with the increase of treating time,while the activity of ProDH was decreased.Gene expression of ProDH was upregulated.[Conclusion]The result showed that ProDH activity was inconsistent with expression of ProDH mRNA.In conclusion,the enzymatic activity of ProDH could be dual controlled by both transcription and translation.

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