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Position: Home > Articles > 具有除草活性的生防菌株GD-9发酵条件优化及菌剂制备 Plant Protection 2019 (3) 88-95

具有除草活性的生防菌株GD-9发酵条件优化及菌剂制备

作  者：
马永强;朱海霞
单  位：
青海大学农林科学院青海大学省部共建三江源生态与高原农牧业国家重点实验室农业部西宁作物有害生物科学观测实验站青海省农业有害生物综合治理重点实验室
关键词：
生防菌株;除草活性;发酵条件;菌剂制备
摘  要：
为了明确具有除草活性的生防菌株GD-9发酵过程中各因子的配比和最优条件,采用单因素试验对菌株最适碳源、氮源、载体、固态发酵基质进行了筛选,应用正交试验设计研究了碳源、氮源、初始含水量、接种量、初始pH、培养时间、培养温度7种因素对菌株活菌数的影响.试验结果表明菌株GD-9最佳固态发酵条件为:氮源NaNO3 58.4 mg/g,碳源葡萄糖48.4 mg/g,培养基质最适初始含水量为258 mg/g,最适接种量为0.26 mL/g,初始pH 7.6,最佳培养时间147.8 h,最佳培养温度30.7℃,最适宜的载体为黏土,分散剂为聚乙烯醇,稳定剂为膨润土,润湿剂为糊精.通过发酵试验结果制备生防菌株GD-9的固体菌剂,该研究为菌剂的商品化生产奠定基础.
作  者：
MA Yongqiang;ZHU Haixia;Academy of Agricultural and Forestry Sciences of Qinghai University;State Key Laboratory of Plateau Ecology and Agriculture of Qinghai University;Scientific Observing and Experimental Station of Crop Pests in Xining, Ministry of Agriculture;Key Laboratory of Agricultural Integrated Pest Management in Qinghai Province;
关键词：
biocontrol strain;;herbicidal activity;;fermentation conditions;;preparation of microbial inoculum
摘  要：
In order to clarify the proportion and optimal conditions of various factors in the fermentation process of biocontrol strain GD-9 with herbicidal activity, the single factor experiment was performed to screen the optimal carbon source, nitrogen source, carrier and solid fermentation substrate of the strain. The orthogonal experiment design was used to study the influences of carbon source, nitrogen source, initial water content, inoculation amount, initial pH value, culture time and culture temperature on the colony number of the strain. The experimental results showed that the optimal solid state fermentation conditions of strain GD-9 were N-source NaNO_3 58.4 mg/g, C-source glucose 48.4 mg/g, 258 mg/g of initial water content of the culture medium, 0.26 mL/g of inoculation amount, the initial pH 7.6, 147.8 h and 30.7℃ of culture time and temperature, respectively. The optimal carrier, dispersant, stabilizer and wetting agent were clay, polyvinyl alcohol, bentonite and dextrin. The solid microbial inoculum of biocontrol strain GD-9 was prepared through fermentation test results. This study laid a foundation for the commercial production of the microbial inoculum.

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