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当前位置: 首页 > 文章 > 长期干旱胁迫下 Mdm-miR160-MdARF17-MdHYL1模块调控苹果生长发育的研究 西北农业学报 2024,33 (3) 490-502
Position: Home > Articles > Regulation of Apple Growth and Development by Mdm-miR160-MdARF17-MdHYL1 Module under Long-term Drought Stress Acta Agriculturae Boreali-occidentalis Sinica 2024,33 (3) 490-502

长期干旱胁迫下 Mdm-miR160-MdARF17-MdHYL1模块调控苹果生长发育的研究

作  者：
刘晨;毛秀山;刘祥梅;申小霞;刘振中
单  位：
;西北农林科技大学园艺学院
关键词：
苹果;长期干旱;MdARF17;Mdm-miR160;MdHYL1
摘  要：
为了研究长期干旱条件下Mdm-miR160-MdARF17-MdHYL1调控网络在苹果生长发育中的作用,以 GL-3 和 Mdm-miR160e OE、MdARF17 RNAi、MdmARF17 OE、MdHYL1 OE 及MdHYL 1 RNAi 转基因苹果为试验材料,进行为期3个月的长期干旱处理,测定苹果株高、根干质量、根冠比以及叶片净光合速率、胞间CO2浓度、气孔导度、蒸腾速率、叶绿素荧光值和水分利用效率.结果表明,在长期干旱胁迫下,Mdm-miR160e OE转基因苹果植株更矮化,根冠比、根干质量、光合能力及水分利用效率大于GL-3植株,具有更强的耐旱性;MdARF17 RNAi植株比GL-3更矮,且具有更大的根冠比、根干质量、光合能力和水分利用效率,而MdARF17 OE与之相反;MdHYL1 RNAi植株对长期干旱胁迫更敏感,其株高大于野生型且具有更小的根冠比、更低的光合能力和水分利用效率,而MdHYL1 OE植物对长期干旱条件具有更强的适应性,生长发育表型与RNAi植株相反.综上所述,Mdm-miR160-MdARF17-MdHYL1调控网络在苹果响应长期干旱过程中发挥了重要功能,为苹果抗旱分子育种提供了候选基因.
译  名：
Regulation of Apple Growth and Development by Mdm-miR160-MdARF17-MdHYL1 Module under Long-term Drought Stress
关键词：
Apple%Long-term drought%MdARF17%Mdm-miR160%MdHYL1
摘  要：
GL-3 and Mdm-miR160e OE,MdARF17 RNAi,MdmARF17 OE,MdHYL1 OE,MdHYL1 RNAi transgenic apples were used as materials to investigate the role of Mdm-miR160-MdARF1 7-MdHYL1 regulatory network in growth and development of apple trees under long-term drought stress,after three months of drought treatment,we measured plant height,root dry mass,root to shoot ratio,and leaf net photosynthetic rate,intercellular CO2 concentration,stomatal conductance,transpiration rate,Fv/Fm and water use efficiency.The results showed that under long-term drought stress,compared with GL-3 plants,Mdm-miR160e OE transgenic apple plants exhibited a more dwarf symptom,with a higher root-to-shoot ratio,root mass,photosynthetic capacity,and water use efficien-cy compared to GL-3 plants.They demonstrated a stronger drought tolerance;MdARF17 RNAi plants were shorter than GL-3,with a higher root to shoot ratio,greater root dry mass,enhanced pho-tosynthetic capacity,and improved water use efficiency.In contrast,MdARF17 OE plants displayed characteristics opposite to those of MdARF17 RNAi lines.MdHYL1 RNAi plants were more sensitive to long-term drought stress,with higher plant height compared to the wild-type,a smaller root-to-shoot ratio,lower photosynthetic capacity,and reduced water use efficiency.On the other hand,MdHYL1 OE plants were better adapted to long-term drought conditions and exhibited phenotypes that were the opposite of those seen in MdHYL1 RNAi lines.Therefore,Mdm-miR160-MdARF17-MdHYL1 regulatory network plays an important role in apple responses to long-term drought stress and provides candidate genes for molecular breeding to enhance drought resistance.

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