Position: Home > Articles > Establishment of a HPLC method for determination of the coupling ratio of the complete antigen MQCA-BSA
Heilongjiang Animal Science and Veterinary Medicine
2012
(23)
67-69
全抗原MQCA-BSA耦联比HPLC测定方法的建立
作 者：
张景艳;杨志强;李建喜;王学智;张凯;孟家仁;王磊
单 位：
中国农业科学院兰州畜牧与兽药研究所甘肃省新兽药工程重点实验室/甘肃省中兽药工程技术研究中心
关键词：
3-甲基喹啉-2-羧酸(MQCA);高效液相色谱(HPLC)法;耦联比
摘 要：
为了建立一种准确、可靠的测定人工抗原耦联比的方法,试验应用高效液相色谱(HPLC)法采用Waters 2695分离系统、2489紫外检测系统,流动相为甲醇、水之比为60∶40,柱温为30℃,流速为1.00 mL/min,检测波长为320 nm,测定全抗原合成中游离3-甲基喹啉-2-羧酸(MQCA)的含量。结果表明:游离MQCA的线性范围为0.2～128.0μg/mL,线性方程为A=17 100 C+16 667(R2=0.999 5)。说明本方法准确、可靠,适用于人工抗原偶联比的测定。
译 名：
Establishment of a HPLC method for determination of the coupling ratio of the complete antigen MQCA-BSA
作 者：
ZHANG Jing-yan,YANG Zhi-qiang,LI Jian-xi,WANG Xue-zhi,ZHANG Kai, MENG Jia-ren,WANG Lei(Key Laboratory of Veterinary Pharmaceutical Development,Ministry of Agriculture,Lanzhou 730050; Lanzhou Institute of Husbandry and Pharmaceutical Sciences,CAAS,Lanzhou 730050;Engineering & Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province,Lanzhou 730050)
关键词：
3-methyl quinoxaline-2-carboxylic acid(MQCA);high performance liquid chromatography(HPLC) method;coupling ratio
摘 要：
To establish an accurate and reliable method for determination of the coupling ratio of artificial antigen,the content of free 3-methylquinoline evil-2-carboxylic acid(MQCA) in the synthesis of the complete antigen was determined by Waters 2695 and 2489 UV HPLC system.In the experiment,the mobile phase was methanol,the water ratio was 60∶ 40,the column temperature was 30 ℃,the flow rate of 1.00 mL/min,and the detection wavelength was set at 320 nm.The results showed that the linear range for the free MQCA were from 0.2 to 128.0 g/mL,and the linear equation was A = 17 100C +16 667(R2 = 0.999 5).The results indicate that the method is accurate,reliable,and suitable for the determination of the coupling ratio of the artificial antigen.
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