作 者：
黄立飞;陈景益;房伯平;罗忠霞;张雄坚;王章英
单 位：
广东省农业科学院作物研究所广东省农作物遗传改良重点实验室
关键词：
甘薯茎腐病;达旦提狄克氏菌;遗传多样性;重复序列PCR基因指纹;致病力差异
摘 要：
为了解不同地区甘薯茎腐病菌Dickeya dadantii种群遗传多样性水平及致病力差异,采用重复序列PCR基因指纹(repetitive element palindromic PCR,REP-PCR)技术和薯片接种方法,对采自广东省、广西壮族自治区和重庆市的6个市区县的59株菌株进行分析。结果表明,5对引物对59株菌株扩增出41个清晰的条带,其中36个为多态性条带,每对引物的扩增条带数在4～10之间,平均为7.2。在物种水平上,有效等位基因数、Nei’s基因多样性指数和Shannon信息指数分别为1.4768、0.2801和0.4186,其中湛江种群多样性最高,南宁种群多样性最低;当遗传相似系数为0.79时,59株菌株可被划分为5个类群,类群划分与菌株来源地间有一定的相关性。此外,不同地区病菌种群间存在明显的致病力差异,其中合浦种群与湛江种群致病力最强,万州种群致病力较弱。表明甘薯茎腐病菌种群具有丰富的遗传多样性,不同地区的病菌种群存在明显的遗传多样性与致病力差异。
译 名：
Pathogenic differentiation and genetic diversity of Dickeya dadantii causing bacterial stem and root rot of sweetpotato
作 者：
Huang Lifei;Chen Jingyi;Fang Boping;Luo Zhongxia;Zhang Xiongjian;Wang Zhangying;Guangdong Provincial Key Laboratory of Crops Genetics and Improvement, Crop Research Institute, Guangdong Academy of Agricultural Sciences;
单 位：
Huang Lifei%Chen Jingyi%Fang Boping%Luo Zhongxia%Zhang Xiongjian%Wang Zhangying%Guangdong Provincial Key Laboratory of Crops Genetics and Improvement, Crop Research Institute, Guangdong Academy of Agricultural Sciences
关键词：
bacterial stem and root rot of sweetpotato;;Dickeya dadantii;;genetic diversity;;repetitive element palindromic PCR;;pathogenic differentiation
摘 要：
To understand the pathogenic differentiation and genetic diversity of Dickeya dadantii causing bacterial stem and root rot of sweetpotato, 59 isolates collected from six different regions in Guangdong Province, Guangxi Zhuang Autonomous Region, and Chongqing City were analyzed by using repetitive-element PCR genomic fingerprinting(REP-PCR) techniques and chip inoculation methods. The results showed that 41 obvious bands were amplified, in which 36 bands were polymorphic. For each primer pair, four-ten alleles were detected with an average of 7.2. At the level of species, effective number of alleles, diversity index of Nei's genes, and Shannon information index were 1.4768, 0.2801 and0.4186, respectively. Genetic diversity of Zhanjiang population was the maximal, and the minimal genetic diversity was found in Nanning population. The genetic similarity coefficient(GSC) was 0.79, and the 59 strains of bacterial stem and root rot of sweetpotato collected from six different regions were divided into five major groups using unweighted pair-group method with arithmetic averages(UPGMA)method according to genetic distance and GSC. The clustering groups based on GSC were associated with geographic origins to some degree. The pathogenic populations collected from different regions were different in infection ability, in which Hepu population and Zhanjiang population had the highest capacity to cause bacterial stem and root rot of sweetpotato, while Wanzhou population had the relative-ly weak pathogenicity. The results suggested that D. dadantii populations had a rich genetic diversity,while there were significant differences between pathogenic populations in different regions. D. dadantii causing bacterial stem and root rot of sweetpotato had wide pathogenic differentiation in different regions.
