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Position: Home > Articles > Determination of Total Alkaloids in Emilia sonchifolia Collected at Different Harvesting Time in Guangxi FOOD SCIENCE 2009,30 (6) 151-154

不同采收期广西一点红总生物碱含量分析

作  者:
周吴萍;李军生;韦媛媛;阎柳娟;梁燕
单  位:
广西工学院生物与化学工程系
关键词:
一点红;总生物碱;苦参碱;含量测定
摘  要:
目的:建立一点红总生物碱的含量测定方法,测定不同采收期一点红中总生物碱含量,考察一点红原药材质量的优劣。方法:利用单因素法考察最佳提取条件,采用紫外可见分光光度法,以苦参碱为对照品,于414nm波长处测定生物碱含量。结果:苦参碱在0.0021~0.0294mg/ml范围内呈良好线性关系(R2=0.9997),平均加样回收率为97.6%,RSD为0.447%(n=6)。样品的总生物碱含量在4.31~13.99mg/g之间,八月为最佳采收期,其样品中总生物碱含量最高。结论:本法测定,重复性较好,对仪器要求不高,准确可靠,可用于一点红质量控制。
译  名:
Determination of Total Alkaloids in Emilia sonchifolia Collected at Different Harvesting Time in Guangxi
作  者:
ZHOU Wu-ping,LI Jun-sheng,WEI Yuan-yuan,YAN Liu-juan,LIANG Yan (Department of Biological and Chemical Engineering, Guangxi University of Technology, Liuzhou 545006, China)
关键词:
Emilia sonchifolia;total alkaloids;matrine;content determination
摘  要:
Objective: To establish a method for determing total alkaloids content in Emilia sonchifolia and then determine its best harvesting time. Methods: The optimum extraction conditions were confirmed by single factor tests, and with matrine as standard, total alkaloids content was determined at the wavelength of 414 nm by UV-visible spectrophotometry. Results: The calibration curve shows good linearity in the range of 0.0021 to 0.0294 mg/ml (R2=0.9997), and the average spike recovery is 97.6% with the RSD of 0.447% (n=6). The contents of total alkaloids in chloroform extract samples of Emilia sonchifolia collected at different harvesting time are 4.31 to 13.99 mg/g, and August is considered as the best harvesting time of Emilia sonchifolia with the highest total alkaloids content. Conclusion: The method is simple and accurate with high repeatability, and can be used for quality analysis of Emilia sonchifolia.

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