Liu Ling-zhi;Qin Si-jun;Lu De-guo;Wang Bing-ying;Yang Ze-yuan

Shenyang Agr Univ, Key Lab Northern Fruit Cultivat & Physioecol Shen, Coll Hort, Shenyang 110866, Peoples R China;Shenyang Agr Univ, Foreign Language Dept, Shenyang 110866, Peoples R China;Shenyang Agr Univ, Coll Land & Environm, Shenyang 110866, Peoples R China

apple orchard soil;ammonia-oxidizing bacteria;potential nitrification;community structure;PCR-denaturing gradient gel electrophoresis

In this study, we investigated the potential nitrification and community structure of soil-based ammonia-oxidizing bacteria (AOB) in apple orchard soil during different growth periods and explored the effects of environmental factors on nitrification activity and AOB community composition in the soil of a Hanfu apple orchard, using a culture-dependent technique and denaturing gradient gel electrophoresis (DGGE). We observed that nitrification activity and AOB abundance were the highest in November, lower in May, and the lowest in July. The results of statistical analysis indicated that total nitrogen (N) content, NH4+-N content, NO3--N content, and pH showed significant correlations with AOB abundance and nitrification activity in soil, The Shannon-Winner diversity, as well as species richness and evenness indices (determined by PCR-DGGE banding patterns) in soil samples were the highest in September, but the lowest in July, when compared to additional sampled dates. The DGGE fingerprints of soil-based 16S rRNA genes in November were apparently distinct from those observed in May, July, and September, possessing the lowest species richness indices and the highest dominance indices among all four growth periods. Fourteen DGGE bands were excised for sequencing. The resulting analysis indicated that all AOB communities belonged to the beta-Proteobacteria phylum, with the dominant AOB showing high similarity to the Nitrosospira genus, Therefore, soil-based environmental factors, such as pH variation and content of NH4+-N and NO3--N, can substantially influence the abundance of AOB communities in soil, and play a critical role in soil-based nitrification kinetics.