Position: Home > Articles > Production of Functional 1,3-Diacylglyerol from Monoacylglycerol by Lipase-Catalyzed Transesterification
FOOD SCIENCE
2015,36
(22)
1-5
脂肪酶催化单油酸甘油酯制备功能性1,3-甘油二酯
作 者：
黄楚楚;熊辉煌;龚斌;喻芸;冯越;梁媛;朱雪梅
单 位：
南昌大学食品学院食品科学与技术国家重点实验室
关键词：
酰基迁移;共轭亚油酸;共轭亚油酸乙酯;单油酸甘油酯;脂肪酶TLIM
摘 要：
研究固定化脂肪酶TLIM催化单油酸甘油酯(glycerol monooleate,GMO)制备1,3-甘油二酯(sn-1,3-diacylglyerol,sn-1,3-DAG)。比较了游离脂肪酸(共轭亚油酸)和脂肪酸乙酯(共轭亚油酸乙酯)两种不同类型酰基供体、反应时间、底物物质的量比对酰基迁移和sn-1,3-DAG的影响。通过对实验结果的判定及分析得到最佳反应条件为采用20%(质量分数)脂肪酶TLIM、底物物质的量比(共轭亚油酸乙酯和GMO)3∶1、在50?℃的220 r/min水浴摇床中反应2 h,最后得到sn-1,3-DAG转化率为65%。本研究利用GMO而不是常规的甘油或者甘油三酯来制备sn-1,3-DAG,并比较了不同酰基供体对酰基迁移和sn-1,3-DAG转化率的影响,旨在为脂肪酶催化法制备功能性sn-1,3-DAG的研究提供一定参考。
译 名：
Production of Functional 1,3-Diacylglyerol from Monoacylglycerol by Lipase-Catalyzed Transesterification
作 者：
HUANG Chuchu;XIONG Huihuang;GONG Bin;YU Yun;FENG Yue;LIANG Yuan;ZHU Xuemei;State Key Laboratory of Food Science and Technology,School of Science and Technology,Nanchang University;
关键词：
transesterification;;conjugated linoleic acid;;conjugated linoleic acid ethyl ester;;glyceryl monooleate;;lipase TLIM
摘 要：
sn-1,3-Diacylglycerol(sn-1,3-DAG) was synthesized from glycerol monooleate(GMO) by the immobilized Thermomyces lanuginosus lipase(Lipozyme TLIM)-catalyzed esterification in a solvent-free system.The effects of different types of acyl donors such as conjugated linoleic acid(CLA-FA) and conjugated linoleic acid ethyl ester(CLA-EE),reaction time and molar ratio of substrates on acyl migration and glycende concentration were investigated to improve the yield of sn-1,3-DAG,of the yield of sn-1,3-DAG was 65%when the reaction proceeded at a TLIM concentration of 20%by mass with a molar ratio of conjugated linoleic acid ethyl ester(CLAEE):GMO of 3:1 for 2 h at 50 ℃ in a water bath shaker at220 r/min.Also,the effects of two types of acyl donors(free acid and ethyl ester) on acyl migration and the yield of sn-1,3-DAG were compared.This study can provide a reference for the industrial production of sn-1,3-DAG.
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