当前位置: 首页 > 文章 > 雷公藤内生细菌的促生作用及其对雷公藤甲素生成的影响 应用生态学报 2014,25 (6) 1681-1687
Position: Home > Articles > Growth-promoting effect and triptolide production regulation of endophytic bacteria from Tripterygium wilfordii Chinese Journal of Applied Ecology 2014,25 (6) 1681-1687

雷公藤内生细菌的促生作用及其对雷公藤甲素生成的影响

作  者:
许进娇;宋萍;封磊;洪伟;吴承祯;李国庆;赵希俊;葛恒懿
单  位:
福建农林大学林学院;福建农林大学资源与环境学院
关键词:
雷公藤;内生细菌;促生;次生代谢;雷公藤甲素
摘  要:
从健康雷公藤植株体内分离得到23株内生细菌,对其有益生物学特征及对小麦萌发能力的影响进行评价,得出3株益生效果显著的内生细菌.这3株内生细菌均能产生植物生长素和铁载体,其中LG3、LJ10具有溶磷特性,LG3、LY4具有固氮功能并同时具有1-氨基环丙烷-1-羧酸脱氨酶(ACC脱氨酶)活性.经细菌16S rDNA序列鉴定及同源性分析发现,LG3、LY4属于肠杆菌属,LJ10属于泛菌属.回接试验表明,3株内生细菌不但能够促进雷公藤植株的生长,还能显著提高不同器官内雷公藤甲素的含量.
译  名:
Growth-promoting effect and triptolide production regulation of endophytic bacteria from Tripterygium wilfordii
作  者:
XU Jin-jiao;SONG Ping;FENG Lei;HONG Wei;WU Cheng-zhen;LI Guo-qing;ZHAO Xi-jun;GE Heng-yi;College of Forestry,Fujian Agriculture and Forestry University;College of Resources and Environment,Fujian Agriculture and Forestry University;
关键词:
Tripterygium wilfordii;;endophytic bacteria;;growth-promotion;;secondary metabolism;;triptolide.
摘  要:
A total of twenty-three endophytic bacteria were isolated from Tripterygium wilfordii,among which three strains were selected for further studying based on their capabilities of growthpromotion and wheat germination. All three isolated strains could produce phytohormone and siderophore,and LG3 and LJ10 strains were capable of dissolving phosphorus. Additionally,LG3and LY4 strains were both proved to have nitrogen-fixing function and 1-aminocyclopropane-1-carboxy-late deaminase activity. Bacterial 16S rDNA sequence identification and homology analysis suggested that LG3 and LY4 strains belonged to the Enterobacter and LJ10 belonged to the Pantoea.The results of reinoculation experiment demonstrated that three endophytic bacteria could not only promote the growth of Tripterygium wilfordii,but also improve the triptolide contents of different organs significantly.

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