作 者：
宋海岩;陈栋;李靖;钟小江;杨文渊;祝进;江国良
单 位：
宜宾县农业局果树站;四川省农业科学院园艺研究所·农业部西南地区园艺作物生物学与种质创制重点实验室;四川省园艺推广总站
关键词：
GF677;毛桃;碱性土;黄化;叶绿素合成;叶绿体
摘 要：
【目的】探究桃砧木GF677与普通毛桃在碱性土上抗黄化生理机制,为抗性桃砧木育种提供理论依据。【方法】以两年生GF677扦插苗和毛桃实生苗为材料,比较其在碱性土上不同时期叶绿素合成前体及组分含量差异。【结果】多数时期GF677叶片叶绿素a、叶绿素b、叶绿素总量及UrogenⅢ、ProtoⅨ、Mg-ProtoⅨ和原脱植基叶绿素含量均高于毛桃; GF677叶片内ProtoⅨ、Mg-ProtoⅨ以及原脱植基叶绿素含量在受到碱胁迫后能在短时间内恢复并超越前期值,而毛桃恢复水平较低。【结论】毛桃叶绿素b含量各时期均极显著低于GF677,是导致毛桃叶片黄化的直接原因;叶片PBG→Uro、Uro→Proto两个合成步骤,及叶绿素循环中叶绿素a→叶绿素b转化是否受阻是桃砧木抗黄化能力的重要判断指标。
译 名：
Chlorophyll Synthesis and Chloroplast Structure Comparison Research between GF677 and Wild Peach on Alkaline Soil
作 者：
SONG Hai-yan;CHEN Dong;LI Jing;ZHONG Xiao-jiang;YANG Wen-yuan;ZHU Jin;JIANG Guo-liang;Horticulture Institute,Sichuan Academy of Agricultural Sciences·Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(Southwest Region) ,Ministry of Agriculture;Fruit Tree Station of Agriculture Bureau of Yibin;Sichuan Horticulture Promotion Station;
关键词：
GF677;;Wild peach;;Alkali-resistant;;Anti-yellow;;Chlorophyll synthesis;;Chloroplast
摘 要：
【Objective】The study is aimed to identify physiological mechanism of anti-yellowing for both common prunus persica and resistant peach rootstocks GF677,and provides a theoretical basis of resistive breeding for peach rootstock. 【Method】Taking biennial GF677 seedlings and peach cuttings living on alkaline soil as research targets,it compares their progresses of chlorophyll biosynthesis precursors at different stages as well as composition variances,and then employs Transmission Electron Microscopy( TEM) to examine the ultra-microstructure of chloroplast. 【Results】It confirmed:( i) At multiple stages, the contents of chlorophyll a,chlorophyll b and the total chlorophyll amount contained in the leaves of GF677,as well as Urogen III,Proto IX,the Mg-Proto IX,and original chlorophyllide,were higher than all those of prunus persica. In the leaves of GF677,Proto Ⅸ,Mg-Proto Ⅸ and original chlorophyllide were interfered by alkaline stress,but it soon recovered from a previous transient drop in these contents and then outdone,whereas peach behaved low performance. As compared with prunus persica,GF677 had a complete chloroplast structure with accurate lamellar arrangement,high starch content; However,in the leaves of prunus persica,lamellar structure was loose or even disintegrative,and in the late stages of development,there was obvious increases in the number of osmiophilic granules in the body of chloroplast and it revealed a senescence condition. 【Conclusion】Peach chlorophyll b content has been always significantly lower than GF677 at any stages,which could be used to explain leaf chlorosis; The synthesis procedure,including the conversion of PBG into Uro,and Uro into Proto,and the conversion of chlorophyll a into chlorophyll b,the process of chlorophyll circulation,all of those were determinant references to capability estimation of yellowing resistance of peach rootstocks.
