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当前位置: 首页 > 文章 > 正交试验优化淫羊藿总黄酮和多糖的分步提取工艺优化 食品科学 2012,33 (24) 56-60
Position: Home > Articles > Orthogonal Array Design for the Optimization of Successive Extraction of Total Flavonoids and Polysaccharides from Epimedium brevicornum Maxim FOOD SCIENCE 2012,33 (24) 56-60

正交试验优化淫羊藿总黄酮和多糖的分步提取工艺优化

作  者：
付亮;袁璟亚;杨瑞武;张利;周永红;丁春邦
单  位：
四川农业大学生命科学与理学院;四川农业大学
关键词：
淫羊藿;总黄酮;多糖;分步提取
摘  要：
通过对淫羊藿总黄酮和多糖进行分步提取工艺优化,探寻一种提高淫羊藿综合利用率的提取工艺。利用单因素结合正交试验优化淫羊藿总黄酮的超声波辅助提取工艺,并对此工艺所得药渣进行多糖的水煮提取工艺优化。结果表明,总黄酮提取的最优工艺条件为按料液比1:10(g/mL)加入70%乙醇溶液,在60℃提取温度、150W超声功率下提取2次,每次5min,提取效率达4.72%;多糖提取的最优工艺条件为按料液比1:10加入水,在90℃提取温度提取2次,每次30min,提取效率达1.89%。优化后的淫羊藿总黄酮和多糖分步提取工艺操作简单、能耗低、提取效率高、多糖损失率低、较大限度地提高了淫羊藿的综合利用率。
译  名：
Orthogonal Array Design for the Optimization of Successive Extraction of Total Flavonoids and Polysaccharides from Epimedium brevicornum Maxim
作  者：
FU Liang1,YUAN Jingya2,YANG Rui-wu1,ZHANG Li1,ZHOU Yong-hong2,DING Chun-bang1,*(1.College of Life Science,Sichuan Agricultural University,Ya’an 625014,China;2.Key Laboratory of Crop Germplasm Resources and Gene Improvement,Ministry of Education,Sichuan Agricultural University,Chengdu 611130,China)
关键词：
Epimedium brevicornum Maxim;total flavonoids;polysaccharides;two-step extraction
摘  要：
Objective: To optimize process conditions for the extraction of total flavonoids from the whole plant of Epimedium brevicornum Maxim and for further extraction of polysaccharides from the remaining residue.Methods: A twostep sequential extraction procedure was proposed for the extraction of total flavonoids by ultrasonic-assisted extraction and polysaccharides by hot water extraction.Results: The optimum conditions for the extraction of total flavonoids were found to be two extraction cycles at 60 ℃ and a microwave power of 150 W for 5 min each cycle with 70% ethanol at a solid-tosolvent ratio of 1:10(g/mL),resulting in an extraction efficiency of 4.72%.The optimum conditions for the extraction of polysaccharides were found to be two extraction cycles at 90 ℃ for 30 min each cycle with a residue-to-water ratio of 1:10 g/mL,resulting in an extraction efficiency of 1.89%.The optimized procedure for sequential extraction of total flavonoids and polysaccharides was characterized by easy operation,low energy consumption,high extraction efficiency,low polysaccharide loss and enhanced comprehensive utilization of Epimedium brevicornum Maxim.

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