关键词：
雪卡毒素;快速溶剂萃取;固相萃取;液相色谱-串联质谱
摘 要：
雪卡毒素中毒是一种全球暴发的海洋食源性疾病,食用含有0.1μg雪卡毒素的珊瑚鱼即可引起中毒。研究建立了一套快速、高效、高灵敏度的ASE-SPE-HPLC/MS/MS检测方法来保证人类健康,该方法在1.0~50.0μg·kg-1范围内线性良好,相关系数在0.99以上,检出限为0.03μg·kg-1,定量下限为0.1μg·kg-1,基质加标的回收率在95%以上。较之传统方法,该方法有较好的回收率和提取效率。
译 名：
Determination of ciguatoxin in coral fish by ASE-SPE-HPLC /MS /MS method
作 者：
ZHOU Xiu-jin;ZHOU Xiang-yang;SHAO Hong-hong;YAN Jian-bo;FU Mi-ni;Zhoushan Entry-exit Inspection and Quarantine Bureau;
关键词：
ciguatoxin;;accelerated solvent extraction;;solid phase extraction;;HPLC-MS /MS
摘 要：
Ciguatera fish poisoning(CFP) was a global food-borne illness caused by consumption of sea food containing ciguatoxin(CTX).P-CTX-1 had been suggested to be the most toxic CTX,which caused ciguatera at 0.1μg in the flesh of carnivorous fish.A sensitive and rapid method for the detection of ciguatoxin in coral fish using ASESPE-HPLC / MS / MS was developed in the study.The calibration curve of P-CTX1 showed good linearity in the range of 1.0-50.0 μg·kg-1with correlation coefficient of 0.99.The validated method had limits of detection(LOD) and quantification(LOQ) of 0.03 μg·kg-1 and 0.1μg·kg-1,respectively,for matrix-based standard solutions,as well as matrix spike recoveries ratio above 95%.Compared with previous methods,this method had better overall recovery and extraction efficiency.
