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当前位置: 首页 > 文章 > 引种紫锥菊中松果菊苷含量的分析测定 北方园艺 2019 (9)
Position: Home > Articles > 引种紫锥菊中松果菊苷含量的分析测定 Northern Horticulture 2019 (9)

引种紫锥菊中松果菊苷含量的分析测定

作  者：
徐世娟;卢海啸;黄华希;陈荣;朱昌叁
单  位：
红河学院理学院
关键词：
紫锥菊;松果菊苷;高效液相色谱
摘  要：
采集云南省蒙自市引种栽培的紫锥菊,以其根、茎、叶和头状花序为试材,采用高效液相色谱(HPLC)方法研究了松果菊苷的定量分析方法,并对建立的分析技术进行方法学考察,运用该定量分析技术对紫锥菊中不同部位的松果菊苷含量进行测定.结果表明:松果菊苷含量在0.021~0.321μg范围内与峰面积之间呈良好的线性关系,平均加标回收率为93.24%,RSD值为1.28%;紫锥菊不同部位松果菊苷含量在0.075%~0.221%范围内,且叶、头状花序中松果菊苷含量显著高于根和茎中含量.建立的松果菊苷测定分析方法定性定量准确、可靠、重现性好,可用于紫锥菊药材中松果菊苷的质量评价;云南省蒙自市引种栽培生产的紫锥菊药材中松果菊苷含量较高,优于国外原产地及国内报道的引种栽培区.
作  者：
XU Shijuan;LU Haixiao;HUANG Huaxi;CHEN Rong;ZHU Changsan;College of Science,Honghe University;College of Biological and Pharmaceutical Sciences,Yulin Normal University;College of Chinese Medicinal Materials,Jilin Agricultural University;Guangxi Zhuang Autonomous Region Forestry Research Institute;
单  位：
XU Shijuan%LU Haixiao%HUANG Huaxi%CHEN Rong%ZHU Changsan%College of Science,Honghe University%College of Biological and Pharmaceutical Sciences,Yulin Normal University%College of Chinese Medicinal Materials,Jilin Agricultural University%Guangxi Zhuang Autonomous Region Forestry Research Institute
关键词：
Echinacea purpurea;;echinacoside;;high performance liquid chromatography(HPLC)
摘  要：
The test materials were the the root,stem,leaf and head inflorescence of Echinacea purpurea,which was introduced and cultivated in Mengzi city,Yunnan Province.The quantitative analysis method of echinacoside in Echinacea purpurea was studied by using the method of high performance liquid chromatography(HPLC).The quantitative analysis method of HPLC of determining echinacoside in Echinacea purpurea were optimized and investigated.The results showed that there was in a good linear relationship with the peak area in the range of 0.021 to 0.321 μg.The average recovery rate was 93.24%,and the RSD value was 1.28%.The content of echinacoside was in the range of 0.075%-0.221% in different parts of Echinacea purpurea,and the content of echinacoside in leaves and capitulum was significantly higher than that in roots and stems.The determination method with good precision and reproducibility was reliable,and could be used for the quality evaluation of echinacoside in Echinacea purpurea.The content of echinacoside in Echinacea purpurea for cultivated in Mengzi city,Yunnan Province was high,which is superior to foreign origin and domestic reported cultivation areas.

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