Position: Home > Articles > Isolation and Alcohol Fermentation Characteristics of Thermotolerant Yeasts
FOOD SCIENCE
2016,37
(19)
128-134
耐高温酵母菌的筛选及其乙醇发酵特性
作 者：
刘超帝;缪礼鸿;陈瑾;刘蒲临
单 位：
武汉轻工大学生物与制药工程学院
关键词：
耐高温酵母菌;生物多样性;26SrDNA;乙醇发酵
摘 要：
采用平板分离法,从29份新鲜采集的土样中分离获得64株耐高温酵母菌株,并对其在高温条件下的乙醇发酵性能进行了分析比较。26S r DNA D1/D2区域序列测定和生理特征分析结果表明,这些酵母菌株在亲缘关系上可归类于6个属7个种,分别为热带假丝酵母(Candida tropicalis)(占总分离株的39.1%)、马克斯克鲁维酵母(Kluyveromyces marxianus)(占23.4%)、东方伊萨酵母(Issatchenkia orientalis)(占29.7%)、季也蒙毕赤酵母(M.guilliermondii)(占1.6%)、Kazachstania bovina(占1.6%)、Candida palmioleophila(占1.6%)和酿酒酵母(Saccharomyces cerevisiae)(占3.1%)。其中,马克斯克鲁维酵母的耐高温能力和乙醇发酵能力最强;40℃条件下发酵72 h后,发酵液中乙醇体积分数最高可达6.56%,显著高于相同条件下其他耐高温酵母的乙醇产量。上述结果表明,马克斯克鲁维酵母在高温乙醇发酵过程中具有明显优势,可作为利用生物质发酵生产乙醇的优良候选菌株。
译 名：
Isolation and Alcohol Fermentation Characteristics of Thermotolerant Yeasts
作 者：
LIU Chaodi;MIAO Lihong;CHEN Jin;LIU Pulin;School of Biology and Pharmaceutical Engineering,Wuhan Polytechnic University;
关键词：
thermotolerant yeast;;biodiversity;;26S r DNA;;alcohol fermentation
摘 要：
In this study,69 thermotolerant yeast strains were isolated from 29 different soil samples,and the fermentation performance of these strains at high temperature was also detected.Based on 26 S r DNA D1/D2 domain sequence analysis and physiological characterization,these strains were phylogenetically related to 7 species in 6 genera,including Candida tropicalis(39.1% of the total yeast strains),K.marxianus(23.4%),I.orientalis(29.7%),M.guilliermondii(1.6%),K.bovina(1.6%),C.palmioleophila(1.6%) and S.cerevisiae(3.1%).C.tropicalis were the most widely distributed species.The best thermotolerant and ethanol fermentation ability were detected in K.marxianus.The ethanol production reached up to 6.56%(V/V) in 72 h at 40 ℃.These results demonstrated that K.marxianus has obvious advantages in ethanol fermentation at high temperature,which makes it a good potential candidate for commercial biomass ethanol production.
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