The Same Proteomics Analysis of Jersey Yak Crossbreeds and Yak Milk

Jersey yak crossbreeds milk%Yak milk%Whey protein%Milk fat globule membrane protein%Proteomics

To analyze the same protein of whey and milk fat globule membrane(MFGM)of jersey yak crossbreeds milk and yak milk by proteomics method.The milk yield of jersey yak crossbreeds was higher than that of yak.If the function of jersey yak crossbreeds milk is like that of yak milk,it will improve the economic level of local herdsmen.Whey and milk fat were separated by centrifugation,and proteins were extracted respectively.The same protein was ob-tained by qualitative and quantitative analysis of protein by tandem mass tag(TMT)technique.GO functional annota-tion,KEGG metabolic pathway,protein interaction and other bioinformatics analysis were performed on the same protein.Result analysis indicated that 651 whey proteins and 990 MFGM proteins were identified in the peak lactation period of jersey yak crossbreeds milk and yak milk by label quantitative proteomics,and 298 whey proteins and 283 MFGM proteins were screened.GO annotation analysis showed that the same proteins of jersey yak crossbreeds milk and yak milk whey were mainly involved in the negative regulation of endopeptidase activity,immune response and immunoglobulin produc-tion.The same proteins of MFGM were mainly involved in the negative regulation of endopeptidase activity and comple-ment activation.The cell components of the same proteins of whey and MFGM in the classical pathway were mainly ex-tracellular space and extracellular region.The molecular functions involved were mainly guanosine 5'-triphosphate(GTP)enzyme activity and GTP binding.KEGG enrichment analysis showed that the main enrichment pathways of the same proteins in jersey yak crossbreeds milk and yak milk whey and MFGM included complement and coagulation cas-cades,phage and protein processing in endoplasmic reticulum.During the peak lactation period,the proteins with higher abundance in the milk of jersey yak crossbreeds and yak showed excellent performance in immunity and promoting nutri-ent absorption.However,the milk yield of jersey yak crossbreeds was higher than that of yak,which would increase the economic income of local herdsmen and provide more valuable plateau dairy products for people.