Antifungal Activity and Chemical Composition Analysis of Essential Oil from Fruit of Chenopodium ambrosioides L.

CHEN Lijun;ZHI Yanan;WANG Guojun;YIN Jian;XIONG Jianwei;SHI Hongzhong;Department of Agricultural Science,Xinyang College of Agriculture and Forestry;

fruit of Chenopodium ambrosioides;;essential oil;;fumigation antifungal activity;;contact antifungal activity;;antifungal mechanism;;GC-MS

In order to evaluate fumigation and contact antifungal activities of essential oil from fruit of Chenopodium ambrosioides,this study tested its fumigation and contact antifungal activities on mycelia growth and spore germination of plant pathogenic fungi,preliminarily researched its antifungal mechanism,and analyzed its chemical constituents by GC-MS.The results showed that the essential oil from fruit of C.ambrosioides had strong activity against plant pathogenic fungi,the fumigation and contact antifungal activities both enhanced as volatile oil dose increased,and mostly weakened with processing time extended.The EC50 of fumigation and contact activities against mycelia growth of three species of plant pathogenic fungi(Alternaria brassicae,Fusarium graminearum,Bipolaris oryzae) were respectively in 17.4—22.1 μL / L and 103.9—241.7 μL / L.The EC50 of fumigation and contact activities against spore germination of Bipolaris oryzae were respectively 28.8 μL / L and 789.3 μL / L.The mycelia cell inclusion of B.oryzae showed exosmosis under the essential oil fumigation stress; its amplitude of relative conductivity increased more with volatile oil dose increased and processing time extended,when the mycelia of B.oryzae were under the essential oil contact stress.The main chemical constituents of essential oil were 2-carene and ascaridole.The results indicated that fumigation antifungal activity of essential oil from fruit of C.ambrosioides was better than contact activity.