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当前位置: 首页 > 文章 > 树鼩肠道菌群多样性与功能预测研究 安徽农业科学 2019,47 (8) 101-103,113
Position: Home > Articles > Study on the Diversity and Function Prediction of Intestinal Flora in Tupaia belangeri Journal of Anhui Agricultural Sciences 2019,47 (8) 101-103,113

树鼩肠道菌群多样性与功能预测研究

作  者：
杨学芳;李波;郑红;曹雪;王利梅
单  位：
昆明医科大学科研实验中心;昆明医科大学研究生院;昆明医科大学
关键词：
树鼩;肠道菌群;16SrRNA;菌株功能
摘  要：
[目的]鉴定树鼩的肠道菌群,分析树鼩肠道菌群多样性和丰度,并进行菌群功能预测.[方法]随机采集3份雄性树鼩粪便样品,提取树鼩粪便中细菌基因组DNA,通过PCR扩增获得16S rRNA V3~V4片段,采用高通量Illumina PE300平台进行测序,利用BIPES以及QIIME分析并比较菌群结构及多样性.最后,通过PICRUS t软件对肠道菌群的功能进行预测.[结果]树鼩肠道粪便细菌有9门20纲33目53属122种208个OTU.门水平上,优势菌群为厚壁菌门(Firmicutes)和变形菌门(Proteobacteria);属水平上,埃希氏杆菌属(Escherichia)、乳酸菌属(Lactobacillus)和肠球菌属(Enterocossus)为优势菌群.PICRUS t功能预测结果表明,肠道细菌编码的大多数基因与代谢相关,其次是基因信息加工、环境信息加工.COG功能分类分析表明肠道微生物基因在"氨基酸的运输和代谢"和"碳水化合物运输与代谢"功能类群的相对丰度较高.[结论]PICRUS t功能预测到树鼩肠道菌群主要与代谢相关,树鼩肠道菌群是研究肠道菌群与代谢类疾病的一种良好试验材料.
译  名：
Study on the Diversity and Function Prediction of Intestinal Flora in Tupaia belangeri
作  者：
YANG Xue-fang;LI Bo;ZHENG Hong;Research Center of Kunming Medical University;Graduate School of Kunming Medical University;Department of Laboratory Animal Science,Kunming Medical University;
关键词：
Tupaia belangeri;;Intestinal flora;;16S rRNA;;Function of strain
摘  要：
[Objective]To identify the intestinal flora of Tupaia belangeri,analyze the diversity and abundance,and predict the function of the flora.[Method]Three male feces samples of T.belangeri were randomly collected,bacterial genomic DNA were extracted from the feces samples of T.belangeri to make PCR amplification.16 S rRNA V3-V4 fragments were obtained.The high-throughput Illumina PE300 platform was used for sequencing,and the structure and diversity of the bacterial flora were analyzed by BIPES and QIIME.The function of intestinal flora was predicted through PICRUS t.[Result]There were 9 phyla,20 phyla,33 orders,53 genera,122 species,208 OTU in the intestinal fecal flora of T.belangeri.Firmicutes and Proteobacteria dominated at the level of phylum.At the level of genus,Escherichia,Lactobacillus and Enterocossus were the dominant flora.The functional prediction results by PICRUS t showed that most genes encoded by gut bacteria were associated with metabolic "metabolism",followed by genetic information processing "based information processing",environmental information processing "environmental information processing".COG gene function classification statistics showed that the relative abundance of intestinal flora in "amino acid transport and metabolism" and "carbohydrate transport and metabolism" were higher.[Conclusion]The function of PICRUS t predicted that the intestinal flora of T.belangeri was mainly related with the metabolism,and the intestinal flora of T.belangeri was a good experimental material for studying intestinal flora and metabolic diseases.

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