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Position: Home > Articles > ITS Analysis of Quarantine Insect Opogona sacchari (Lepidoptera: Hieroxestidae) from Different Hosts Scientia Silvae Sinicae 2006,42 (6) 144-147

不同寄主上的蔗扁蛾ITS分析

作  者:
沈杰;高其康;沈幼莲;楼兵干
单  位:
浙江省森林植物防治检疫站;浙江大学;浙江省慈溪市森林植物检疫站
关键词:
蔗扁蛾;内转录间隔区;寄主;检疫
摘  要:
The internal transcribed spacer (ITS) of Opogona sacchari, a important quaratine pest, from different hosts was studied by using the special primers designed by the highly conserved region sequence of 18S and 28S rDNA region, and the ITS sequences of Opogona sacchari from diffirent hosts were compared. The total length of O. sacchari ITS1, ITS2 and 5.8S rDNA is 987 bp. The average of G+C contents is 47.2%. Length of ITS1 and ITS2 fragment is 443 bp and 358 bp. The ITS1 and ITS2 from different hosts are identical or unsignificantly difference.
译  名:
ITS Analysis of Quarantine Insect Opogona sacchari (Lepidoptera: Hieroxestidae) from Different Hosts
作  者:
Shen Jie~1 Gao Qikang~2 Shen Youlian~3 Lou Binggan~2(1. Forest Pest Control and Quarantine Station of Zhejiang Province Hangzhou 310004; 2. Zhejiang University Hangzhou 310029;3. Cixi Forest Pest Control Station of Zhejiang Cixi 315300)
关键词:
Opogona sacchari, internal transcribed spacer, host, quarantine
摘  要:
The internal transcribed spacer (ITS) of Opogona sacchari, a important quaratine pest, from different hosts was studied by using the special primers designed by the highly conserved region sequence of 18S and 28S rDNA region, and the ITS sequences of Opogona sacchari from diffirent hosts were compared. The total length of O. sacchari ITS1, ITS2 and 5.8S rDNA is 987 bp. The average of G+C contents is 47.2%. Length of ITS1 and ITS2 fragment is 443 bp and 358 bp. The ITS1 and ITS2 from different hosts are identical or unsignificantly difference.

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