Position: Home > Articles > Construction of Expressing Vector pG-Lfn of Antimicrobial Peptide Lactoferricin
FOOD SCIENCE
2007,28
(7)
286-290
抗菌肽Lactoferricin表达载体pG-Lfn的构建
作 者：
李铁晶;李景鹏;周东坡
单 位：
黑龙江大学生命科学学院;东北农业大学食品学院
关键词：
Lactoferricin;抗菌肽;表达载体
摘 要：
Lactoferricin是一种天然抗菌肽。本实验进行了Lactoferricin基因的PCR合成,将其引入质粒pGEX-6p-1,转化E.coli JM-109,并进行PCR鉴定、酶切鉴定及测序,成功构建了Lactoferricin基因表达载体pG-Lfn。
译 名：
Construction of Expressing Vector pG-Lfn of Antimicrobial Peptide Lactoferricin
作 者：
LI Tie-jing1,LI Jing-peng1,ZHOU Dong-po2, (1.College of Food Science, Northeast Agricultural University, Harbin 150030, China; 2.College of Life Science, Heilongjiang University, Harbin 150030, China)
关键词：
Lactoferricin;antimicrobial peptide;expressing vector
摘 要：
Lactoferricin is a kind of natural antibacterial peptide. In this study, the gene of Lactoferricin was synthesized with PCR, and was linked with the plasmid of pGEX-6p-1, then transformed into E.coli JM-109. The identifications of PCR, enzymical degestion and sequencing were carried out, and the expressing vector of pG-Lfn was successfully constructed.
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