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Position: Home > Articles > STUDIES ON RELATIONSHIPS OF Bursaphelenchus xylophilus AND B .mucronatus BY RAPD Acta Phytopathologica Sinica 2001,31 (3) 225-229

利用RAPD研究松材线虫和拟松材线虫亲缘关系

作  者:
汪来发;王扬;杨宝君;胡先奇;喻盛甫
单  位:
云南农业大学;中国林业科学研究院
关键词:
松材线虫;拟松材线虫;RAPD;亲缘关系
摘  要:
用 RAPD技术对 18个松材线虫株系、8个拟松材线虫株系基因组 DNA多态性进行研究。在事先优化的条件下 ,用 15个随机引物进行 PCR扩增 ,一共扩增出 16 5条谱带 ,多态性为 91.5 %。通过聚类分析 ,结果表明 :松材线虫和拟松材线虫种间和种内都表现出很大的差异性 ,但种间差异大于种内差异 ,因而松材线虫和拟松材线虫应为 2个独立的种。
译  名:
STUDIES ON RELATIONSHIPS OF Bursaphelenchus xylophilus AND B .mucronatus BY RAPD
作  者:
WANG Lai fa 1, WANG Yang 2,YANG Bao jun 1,HU Xian qi 2,YU Sheng fu 2 ( 1 Chinese Academy of Forestry, Beijing 100091; 2 Yunnan Agricultural University, Kunming 650201,China)
关键词:
Bursaphelenchus xylophilus ; B.mucronatus; RAPD; relationship
摘  要:
Random amplified polymorphic DNA (RAPD) technique was applied to assess DNA polymorphism among 18 strains of B. xylophilus and 8 strains of B .mucronatus. Amplifications with 15 primers under predetermined optimal reaction conditions gave 165 reproducible bands and the frequency of genetic diversity was 91.5%. By cluster analysis, the results showed that there was significant genetic diversity between B. xylophilus and B.mucronatus, which interspecific variations were more than intraspecific variations. Thus B. xylophilus and B.mucronatus are two distinct species.

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