Position: Home > Articles > Establishment of determination method of Berberine hydrochloride in veterinary Baitouweng Granules
Animal Husbandry & Veterinary Medicine
2014,46
(7)
6-9
兽用白头翁颗粒中盐酸小檗碱含量测定方法的建立
作 者：
张颖;王艳萍;张志美;徐倩倩;杨丽梅;马力;王建军;郭时金;沈志强
单 位：
山东省滨州畜牧兽医研究院
关键词：
白头翁颗粒;盐酸小檗碱;含量测定;高效液相色谱法
摘 要：
采用ZORBAX.SB-C18(4.6 mm×150 mm,5μm)为色谱柱,柱温30℃,流动相为乙腈-0.05 moL/L磷酸二氢钾溶液(50∶50)(每100 mL中加十二烷基硫酸钠0.4 g,以磷酸调节pH值为4),流速为1.0 mL/min,检测波长342 nm,进样量10μL。结果显示:盐酸小檗碱在0.1~2.5 mg/mL范围内呈良好的线性关系(r=0.999 95,n=7),平均含量为6.23 mg/g,平均加样回收率99.33%(n=9,RSD=0.82%),盐酸小檗碱的定量限为1.0 ng/mL,检测限为0.15 ng/mL。本方法简便、快速、准确、重现性好,可用于兽用白头翁颗粒中盐酸小檗碱的含量测定。
译 名：
Establishment of determination method of Berberine hydrochloride in veterinary Baitouweng Granules
作 者：
ZHANG Ying;WANG Yanping;ZHANG Zhimei;XU Qianqian;YANG Limei;MA Li;WANG Jianjun;GUO Shijin;SHEN Zhiqiang;Shandong Binzhou Animal Science & Veterinary Medicine Institute;Shandong Lvdu Ante Animal Pharmaceutical Co.,LTD;
关键词：
Baitouweng granules;;Berberine hydrochloride;;content determination;;HPLC
摘 要：
This study aims to establish a method for determining Bererine hydrochloride in veterinary Baitouweng granules. The determination was performed on the ZORBAX. SB- C18( 4. 6 mm × 150 mm,5 μm) column with the condition of the mobile phase consisted of acetonitrile- 0. 05 moL /L KH2PO4( adjusted to pH =4 with phosphoric acid)( 50∶ 50,v/v) at the flow rate of 1.0 mL·min- 1,column temperature of 30 ℃ and detection wavelength of 342 nm. The injection volume was 10 μL. The results showed that the linear range of Bererine hydrochloride was 0. 1 ~ 2. 5 mg /mL. The average content of bererine was of 6. 23 mg /g. The average recovery was 99. 33%( n = 9,RSD = 0. 82%). The LOQ and LOD of Berernine hydrochloride were 1. 0 ng /mL and 0. 15 ng /mL,respectively,The results indicated that the method is simple,rapid and accurate with a good reproducibility and can be used for determining the concentration of Bererine hydrochloride in veterinary Baitouweng granules.
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