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当前位置: 首页 > 文章 > 土壤中大豆根瘤菌之间竞争结瘤的研究——Ⅰ、免疫荧光抗体技术在根瘤菌个体生态学研究中的应用 华中农业大学学报 1985 (3) 38-47
Position: Home > Articles > STUDIES ON COMPETITION FOR NODULATION BETWEEN SOYBEAN RHIZOBIA IN SOIL——Ⅰ. APPLICATION OF IMMUNOFLUORESCENT TECHNIQUE TO AUTECOLOGICAL STUDY OF RHIZOBIA Journal of Huazhong Agricultural University 1985 (3) 38-47

土壤中大豆根瘤菌之间竞争结瘤的研究——Ⅰ、免疫荧光抗体技术在根瘤菌个体生态学研究中的应用

作  者：
王福生;陈华癸;李阜棣
单  位：
华中农学院生物固氮研究室
摘  要：
本文详细介绍了免疫荧光技术,并报道了这一技术应用于根瘤菌的个体生态学的研究结果。
译  名：
STUDIES ON COMPETITION FOR NODULATION BETWEEN SOYBEAN RHIZOBIA IN SOIL——Ⅰ. APPLICATION OF IMMUNOFLUORESCENT TECHNIQUE TO AUTECOLOGICAL STUDY OF RHIZOBIA
作  者：
Wang Fusheng;Chen Huakui;Li Fudi
摘  要：
We detailed the immunofluorescent technique and Proved that the fluorescentantibody (FA) technique makes possible an autecological approach to rhizobialecology. The results indicated that FA-counts significantly related with viable platecounts (r=0. 968). The efficient of FA-counts was 30%. Population of indigenoussoybean rhizobia inuninoculated fallow soil remained at initial level(10~4 to 10~5/g)during the period of the field experiment. Comparably significant rhizospherestimulation was observed with field soybean. Nitrogen treatment had a littleeffect on rhizobia densities in soybean rhizosphere. Population of inoculantstrain PRC 005 decreased slightly in field soil during the first 10 days afterinoculation then the P0pulation increased gradually in rhizosphere, reachinga higher level than that in inoculum, with higher rates of inoculation, thepopulation of nodules formed by the inoculant strain was greater. when inoculumrate of 120 times the indigenous population (per g soil)in a pot experimentarranged in the field the nodule occupancy of the inoculant strain was 50. 1% atflowing (55 days after planting).

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