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Position: Home > Articles > Qualitative Analysis of Oligomeric Procyanidins from Litchi chinensis Pericarp FOOD SCIENCE 2012,33 (17) 40-43

荔枝皮原花青素低聚体的定性分析

作  者:
周玮婧;隋勇;孙智达;谢笔钧
单  位:
华中农业大学食品科学技术学院;武汉市食品药品检验所
关键词:
荔枝皮;原花青素;低聚体;LC-MS
摘  要:
制备并定性分析荔枝皮原花青素低聚体(LPOPC)。荔枝皮原花青素(LPPC)通过乙醇浸提,AB-8大孔树脂纯化,Toyopearl HW-40S树脂层析分级处理得到LPOPC,并按聚合度分成了3个组分。通过红外光谱、高效液相色谱(HPLC)和液相色谱-质谱联用(LC-MS)等分析表明,LPOPC中主要成分为(-)-表儿茶素,并且含有A型原花青素二聚体和A型原花青素三聚体。通过选择性的级分收集,可以得到纯度较高的原花青素单体、二聚体和三聚体。
译  名:
Qualitative Analysis of Oligomeric Procyanidins from Litchi chinensis Pericarp
作  者:
ZHOU Wei-jing 1,2,SUI Yong 2,SUN Zhi-da 2,XIE Bi-jun 2(1.Wuhan Institute for Food and Drug Control,Wuhan 430012,China;2.College of Food Science and Technology,Huazhong Agricultural University,Wuhan 430070,China)
关键词:
Litchi chinensis pericarp;procyanidins;oligomer;LC-MS
摘  要:
Litchi(Litchi chinensis Sonn.) is a subtropical fruit of high commercial value;however,its pericarp results in a reduced market value.In the present study,oligomeric procyanidins from Litchi chinensis pericarp(LPOPC) were prepared and qualitatively analyzed.LPOPC were extracted with ethanol aqueous solution,purified by AB-8 macroporous resin chromatography,and fractionated by Toyopearl HW-40S resin chromatography to obtain LPOPC.LPOPC was found to comprise three fractions with different degrees of polymerization(DP).The results of FT-IR,HPLC and LC-MS analyses revealed that the major components of LPOPC were(-)-epicatechin,procyanidin A2,procyanidin A3.Highly pure monomer,dimmers and trimers were obtained through selective collection of fractions.

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