Position: Home > Articles > The Isolation and Identification of an Efficient Fe/Mn-oxidizing Bacterial Strain P1,and the Optimization of Its Oxidizing Conditions
Biotechnology Bulletin
2016,32
(4)
175-183
一株高效铁锰氧化细菌P1的分离鉴定及氧化条件优化
作 者：
樊星;王淑婷;李春艳
单 位：
东北农业大学资源与环境学院
关键词：
铁锰氧化;Bacillus cereus;分离鉴定;氧化条件;响应面分析法
摘 要：
利用富集培养技术从富含铁锰的地下水井淤泥中分离得到1株能够氧化铁锰的细菌,命名为P1。经形态特征、生理生化特征和16SrDNA序列分析,将菌株P1鉴定为蜡状芽孢杆菌(Bacilluscereus)。利用单因素实验探讨菌株P1的生长及氧化特性;采用响应面分析方法考察接种量、温度、pH值3个因素对菌株P1氧化特性的影响,进一步优化菌株的氧化条件。结果表明,菌株P1的最佳氧化条件:温度28.54℃,pH7.23,接种量4.35%。在此条件下,菌株P1在锰含量为200mg/L、铁含量为800mg/L的选择性培养液中培养3d后,锰氧化率达93%以上,铁氧化率达100%。
译 名：
The Isolation and Identification of an Efficient Fe/Mn-oxidizing Bacterial Strain P1,and the Optimization of Its Oxidizing Conditions
作 者：
FAN Xing;WANG Shu-ting;LI Chun-yan;College of Resource and Environment,Northeast Agricultural University;
关键词：
Fe/Mn-oxidizing;;Bacillus cereus;;isolation and identification;;oxidation conditions;;response surface methodology
摘 要：
By enrichment culture,a Fe/Mn-oxidizing bacterial strain P1 was isolated from the sludge samples of groundwater well that was rich in Fe/Mn. According to morphologic and physiological-biochemical characteristics as well as 16 S r DNA sequence analysis,strain P1 was identified as Bacillus cereus. Concurrently,single factor test was used to study the growth of strain P1 and its oxidation characteristics;and the response surface methodology(RSM)was employed to explore the effects of inoculation size,temperature and p H on the oxidation characteristics of strain P1 and further optimize the oxidation conditions. The results showed that the optimal oxidation conditions were temperature 28.54℃,p H 7.23,and inoculation size 4.35 %. At the optimal conditions,the removal ratios of Mn and Fe were 93 % and 100 % respectively in the selective medium containing 200 mg/L Fe and 800 mg/L Mn after strain P1 was cultured for 3 d.
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