Occurrence of Rice Stripe Virus on Wheat in Jiangsu Province and the Identification of Its Pathogen with RT-PCR

CHENG Zhao-bang,DENG Jin-hua,REN Chun-mei,XIONG Ru-yi, ZHOU Tong,FAN Yong-jian,ZHOU Yi-jun(Institute of Plant Protection,Jiangsu Academy of Agricultural Sciences,Nanjing,Jiangsu 210014,China)

Wheat stripe virus disease;Small brown planthopper;Rice stripe virus;Epidemic

A new wheat virus disease was reported in Jiangsu province,China.Wheat plants infected showed chlorotic or yellow stripes which was continuous or discontinuous on the leaves,the diseased new leaf became yellow or seared and the spike was unseeded.The symptoms developed when the lowest day temperature was above 15℃ for a week.Infected plants were mainly found in field where wheat intercroping with rice.The severity of this disease was correlated to the size of the population of the small brown planthopper(Laodelphax striatellus Fallén) overwinter in wheat field.Infected leaves were reacted with antibody of rice stripe virus(RSV) by Dot-ELISA method.Two prospective DNA fragments were successfully amplified from infected wheat leaf by RT-PCR method with primers based on the sequence of RSV-NS3、CP gene from Japanese isolate T.The CP and NS3 gene sequences of Yandu isolate from wheat had more than 95% identities on nucleotides to the RSV Hongze isolate and Japanese T isolate from rice,and had more than 97% identities to the RSV isolate from the small brown planthopper on the same area.The results showed that the pathogen of this disease was Rice stripe tenuivirus.We named this disease as wheat stripe virus disease.