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当前位置: 首页 > 文章 > 孔石莼(Ulva pertusa)中一种抗TMV活性蛋白的纯化及其特性(英文) 植物病理学报 2005,35 (3) 256-261
Position: Home > Articles > Purification and Characterization of an Anti-TMV Protein from a Marin Algae Ulva pertusa Acta Phytopathologica Sinica 2005,35 (3) 256-261

孔石莼(Ulva pertusa)中一种抗TMV活性蛋白的纯化及其特性(英文)

作  者：
刘振宇;谢荔岩;吴祖建;林奇英;谢联辉
单  位：
福建农林大学植物病毒研究所
关键词：
孔石莼;阳离子交换层析;蛋白纯化;抗病毒;TMV
摘  要：
采用硫酸铵盐析和阳离子交换柱层析(CM-SepharoseFastFlow),从孔石莼(UlvapertusaKjellm)藻体中分离纯化得到1个蛋白,命名为UPCM40。经SDS-PAGE确定其分子量约为36kD,Native-PAGE可知其为单一组分;该蛋白不含糖;其全波长扫描结果显示,该蛋白在190~220nm和250~300nm处有特征吸收峰,在250~300nm范围中的最大吸收峰在270~275nm处。经测定发现该蛋白具较好的抗烟草花叶病毒(Tobaccomosaicvirus,TMV)的活性,当蛋白质浓度为50μg/mL时,对TMV的抑制效果为:在枯斑寄主心叶烟上的侵染抑制率达85·6%,在苋色藜上为90·2%。测定该蛋白对6种供试真菌的抑制效果发现,对镰刀菌(Fusariumoxysporumf.sp.cucumerinum)、立枯丝核菌(Rhizoctoniasolani)和香蕉炭疽菌(Gloeosporiummusarum)均有一定程度的抑菌作用,但抑制活性很低。
译  名：
Purification and Characterization of an Anti-TMV Protein from a Marin Algae Ulva pertusa
作  者：
LIU Zhen-yu 1,2, XIE Li-yan 1,WU Zu-jian 1, LIN Qi-ying 1*,XIE Lian-hui 1 ( 1Institute of Plant Virology,Fujian Agriculture and Forestry University,Fuzhou 350002, China; 2College of Plant Protection,Shandong Agricultural University,Tai'an 271018,China)
关键词：
protein;antivirus;Ulva pertusa Kjellm; purification;positive ion-exchange column chromatography;TMV
摘  要：
An antiviral protein UPCM40 was isolated and purified from the algae Ulva pertusa Kjellm through ammonium sulfate precipitation and CM-Sepharose FF ion-exchange column chromatography. It was shown that this kind of protein is a single band with molecular weight of about 36 kD via SDS-PAGE electrophoresis. And it is of only single component under PAGE analysis. Whole waves scanning on UPCM40 showed characteristic peaks at 190-220 nm and 250-300 nm, and the maximum absorption on latter peak was 275 nm. The activity of UPCM40 against the infection of Tobacco mosaic virus(TMV) was tested. The result showed that the inhibition rates were 85.6% and 90.2% on Nicotiana glutinosa and Chenopodium amaranticolor respectively. It also showed that the UPCM40 had certain inhibition activity to Fusarium oxysporum f. sp. cucumerinum, Rhizoctonia solani and Gloeosporium musarum at the concentration of 150 μg/mL through Dual-culture experiments.

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