SHI Jiang;XIN Li;YANG Yan;ZHANG Xue-qin

College of Agronomy; Luoyang 471003; Henan University of Science and Technology

Piper methysticum;Pepper;Amplified fragment length polymorphism

The aim of the research was to discuss the genetic relationships between Piper methysticum, Pepper and other wild species in Pepper genus. DNA was extracted from leaves which belonged to 28 germplasms including 6 materials of P. methysticum, 21 materials of cultivated and wild Pepper, 1 material of Peperomia pellucida belonged to different genus. Premiers with good band-type and high polymorphism and resolution were selected from 64 pairs of primers for AFLP amplification and the clustering analysiswas conducted with MVSP3. 13f software. 191 bands were amplified by 4 pairs of premiers, 189 of which had polymorphism, being 98.6%. 28 germplasms were classified into 6 different groups at the genetic similarity coefficient of 0.52 by silver staining AFLP, in which 6 materials of Piper methysticum were clustered into a single group, indicating that P. methysticum belonged to Pepper family of Pepper genus but were distantly related to the others. The research provided the basis for selecting rootstocksfor P. methysticum graft, molecular identification of P. methysticum and the fingerprint construction of P. methysticum.