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Position: Home > Articles > Laboratory Experiment Optimization of Desalting and De-protein Process for Enteromorpha Polysaccharides Food and Nutrition in China 2012,18 (04) 59-63

浒苔多糖脱蛋白脱盐方法的优选

作  者：
陈达妙;林文庭
单  位：
福建医科大学公共卫生学院营养与保健医学系
关键词：
浒苔多糖;脱蛋白;脱盐;酶法;透析法
摘  要：
目的:探讨浒苔多糖脱蛋白脱盐的方法。方法:利用蛋白酶水解浒苔多糖的蛋白质,通过正交试验优选蛋白酶水解工艺条件;利用透析袋流水透析脱盐,通过正交试验优选透析工艺条件。结果:中性蛋白酶脱蛋白效果优于Sevag法和三氯乙酸法,中性蛋白酶脱蛋白较佳工艺条件为:加酶量0.12g/10mL、pH6.0、反应温度45℃、反应时间1h,在此条件下,蛋白质脱除率达52.36%。透析脱盐的较佳条件为:7000Da、透析时间8h、浒苔多糖溶液浓度6%,在此条件下,灰分脱除率为76.95%。(除杂后,浒苔多糖蛋白质含量为3.43%,灰分含量为8.04%,多糖损失率为18.96%)。结论:蛋白酶法脱蛋白质技术和透析袋流水透析脱盐技术,对浒苔多糖的脱蛋白脱盐效果较好,有一定的推广潜力。
译  名：
Laboratory Experiment Optimization of Desalting and De-protein Process for Enteromorpha Polysaccharides
作  者：
CHEN Da-miao,LIN Wen-ting (Department of Nutrition and Health Care,College of Public Health,Fujian Medical University,Fuzhou 350004,China)
关键词：
enteromorpha polysaccharides;de-protein;desalting;enzymatic method;dialysis method
摘  要：
【Objective】 To study the method of de-protein and desalting process for enteromorpha polysaccharides.【Method】The best method of enzymatic de-protein process was screened from amino-acid nitrogen.The proper method of run water dialysis was chosen from dry ash content and polysaccharides loss rate.【Result】De-protein effect of neutral protease was better than that of papain method,Sevag method and trichloroacetic acid method.The best condition of neutral protease de-protein process was adding 0.12g/10mL of lipase amounts into the extracting liquid,adjusting the pH to 6.0,then keeping it in the constant temperature bath 45℃ for 1h.The proper method of run water dialysis was keeping 6% enteromorpha extracting liquid in the 7?000Da dialysis bag for 8h.The protein content of purer enteromorpha polysaccharides was 3.43%,ash content was 8.04%,and polysaccharides loss rate was 18.96%.【Conclusion】 Neutral protease method and of run water dialysis method are the better ways for de-protein effect and desalting process,which can be used universally.

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