当前位置: 首页 > 文章 > 几种引物对苏铁共生蓝细菌的DNA多态性分析 福建农业学报 2001,16 (4) 54-58
Position: Home > Articles > DNA diversity analysis on the Cyanobacteria freshly isolated from Cycads based on PCR with different primers Fujian Journal of Agricultural Sciences 2001,16 (4) 54-58

几种引物对苏铁共生蓝细菌的DNA多态性分析

作  者:
宋铁英;陈坚;包晓东;郑伟文;郑芳勤
单  位:
福建省农业科学院红萍研究中心;福建省农业科学院生物技术中心;福州国家森林公园
关键词:
苏铁;蓝细菌;DNA多态性;PCR;引物
摘  要:
选用 2种 STRR引物和 4种 Hip引物对 15个苏铁品种的 2 5个苏铁共生蓝藻样品进行 PCR指纹图谱分析 ,通过对这 6种引物分析结果的比较 ,表明 STRR引物 6 80 5 1能较好揭示苏铁共生蓝藻的 DNA多态性 ,试验结果也表明苏铁共生蓝藻和苏铁的共生并非专一
译  名:
DNA diversity analysis on the Cyanobacteria freshly isolated from Cycads based on PCR with different primers
作  者:
SONG Tie\|ying 1,CHEN Jian 2,BAO Xiao\|dong 1, ZHENG Wei\|wen 1, ZHENG Fang\|qin 3 (1.Biotech nology Center,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian\ 350003,China; 2.Azolla Research Center,Fujian Academy of Agricultural Sciences,Fuz
关键词:
Cycads; Cyanobacterium; DNA diversity;PCR; Primer
摘  要:
DNA diversity of the 25 Cyanobacteria freshly isolated from 15 Cycas species were analyzed based on PCR fingerprinting with STRR and Hip primers. In comparison with other 5 primers, STRR primer 68051 was shown to be the best one to distinguish the PCR products of Cyanobacteria in present study. The results indicated that there was no high specificity occurred in the symbiosis between Cycads and its symbionts.

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