当前位置: 首页 > 文章 > 生防菌株XF-1的鉴定和抑菌谱的测定 云南农业大学学报(自然科学) 2009,24 (2) 190-194
Position: Home > Articles > Identification and Fungistatic Effect of a Biocontrol Strain,XF-1 Journal of Yunnan Agricultural University(Natural Science) 2009,24 (2) 190-194

生防菌株XF-1的鉴定和抑菌谱的测定

作  者:
熊国如;赵更峰;范成明;何月秋
单  位:
云南农业大学;河南师范大学
关键词:
枯草芽孢杆菌XF-1;鉴定;十字花科根肿病;生物防治
摘  要:
依据传统形态学、生理生化特征及16S rRNA基因序列,将一株对十字花科根肿病有很好防治效果的菌株XF-1鉴定为枯草芽孢杆菌。采用细菌的通用引物P0,P6扩增16S rRNA基因片段,得到1513 bp的DNA片段,其序列与枯草芽孢杆菌的序列的同源性高达99%。与病原真菌对峙培养的结果表明,该菌株能对供试的卵菌、子囊菌、担子菌和半知菌的21个植物病原真菌均有很好的抑制作用。
译  名:
Identification and Fungistatic Effect of a Biocontrol Strain,XF-1
作  者:
XIONG Guo-ru1,ZHAO Geng-feng2,FANG Cheng-ming1,HE Yue-qiu1,3(1.Key Laboratory of Agricultural Biodiversity and Pests Control,Ministry of Education,Yunnan Agricultural University,Kunming 650201,China;2.College of Life Sciences,Henan Normal University,Xinxiang 453007,China;3.College of Agronomy and Biotechnology,Yunnan Agricultural University,Kunming 650201,China)
关键词:
Bacillus subtilis XF-1;identification;clubroot of cruciferous crops;biological control
摘  要:
A biocontrol strain,XF-1,was identified as Bacillus subtilis based on morphology,physiology,biochemistry and biotechnology,which could control clubroot of cruciferous crops effectively.The sequence of 16S rRNA gene has 99% identity to B.subtilis according to the amplified fragment of 1513 bp with a pair of universal primers,P0 and P6,of bacteria.On co-culture plates this strain showed high suppression effect on 21 fungal pathogens involved in oomycetes,ascomycetes,basidiomycetes and deuteromycetes.

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