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当前位置: 首页 > 文章 > 荔枝胚败育差异表达基因cDNA片段的克隆及序列分析 园艺学报 2004,31 (1) 25-28
Position: Home > Articles > Cloning and Sequencing of cDNA Fragments Differentially Expressed between Aborted and Normal Development Embryo in Litchi Acta Horticulturae Sinica 2004,31 (1) 25-28

荔枝胚败育差异表达基因cDNA片段的克隆及序列分析

作  者：
张以顺;向旭;傅家瑞;黄上志
单  位：
广东省农业科学院果树研究所;中山大学生命科学学院
关键词：
荔枝;败育胚;抑制消减杂交(SSH);Virtual Northern blot
摘  要：
利用抑制消减杂交 (Suppressivesubtractionhybridization ,SSH)克隆荔枝败育胚的差异表达基因cDNA片段。分别以荔枝‘桂味’正常发育胚为driver (驱赶子 ) ,败育胚为tester (检测子 ) ,建立差异表达cDNA文库 ,代表桂味败育胚特异表达的cDNA。经VirtualNorthern检测 ,在桂味正常发育胚 /败育胚差异表达cDNA文库中得到 3个阳性克隆 ,序列分析表明这 3个克隆对应的两个序列在荔枝中为首次报道
译  名：
Cloning and Sequencing of cDNA Fragments Differentially Expressed between Aborted and Normal Development Embryo in Litchi
作  者：
Zhang Yishun 1,Xiang Xu 2,Fu Jiarui 1,and Huang Shangzhi 1 ( 1Life Science School,Zhongshan University,Guangzhou 510275,China; 2Pomology Institute,Guangdong Academy of Agricultural Science,Guangzhou 510640,China)
关键词：
Litchi;Aborted embryo;Suppressive subtraction hybridization(SSH);Virtual Northern blot
摘  要：
The suppressive subtraction hybridization(SSH)technique was used to clone the cDNA fragments differentially expressed between aborted and normal development embryo of‘Guiwei’varieties. One positive differential expressing cDNA library were constructed using cDNAs of normal development embryo of Guiwei as driver and the cDNAs of aborted embryo of Guiwei varieties as tester.This cDNA library indicated the differential expressing cDNA fragments in Guiwei aborted embryo and Guiwei normal development embryo.By Virtual Northern blots,3 positive clones were obtained.Subsequently sequencing analysis showed the 3 clones were corresponding 3 knew gene sequence and the 3 cDNA fragments were first reported in litchi varieties.

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