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当前位置: 首页 > 文章 > 氰氨化钙土壤消毒对黄瓜根腐病及土壤病原菌的控制效果 园艺学报 2016,43 (11) 2173-2181
Position: Home > Articles > Studies of Soil Improvement Effect of Calcium Cyanamide and Its Control Efficiency on Soil-borne Diseases of Vegetable Crops Acta Horticulturae Sinica 2016,43 (11) 2173-2181

氰氨化钙土壤消毒对黄瓜根腐病及土壤病原菌的控制效果

作  者：
贲海燕;石延霞;谢学文;柴阿丽;李宝聚
单  位：
黑龙江省农业科学院园艺分院;中国农业科学院蔬菜花卉研究所
关键词：
黄瓜;氰氨化钙;茄病镰刀菌;防治效果;Real-time PCR;动态监测
摘  要：
应用氰氨化钙土壤消毒技术防治黄瓜根腐病,通过病害防治效果、植株生长情况等指标,结合土壤中茄病镰刀菌实时荧光定量PCR检测手段明确氰氨化钙不同剂量处理后病原菌菌量的动态变化,以此评估氰氨化钙土壤消毒防治黄瓜根腐病的施用效果。应用建立优化的荧光定量PCR检测体系监测土壤中茄病镰刀菌带菌量,最低检测量为1×101个拷贝·g~(-1)。研究结果表明,氰氨化钙最佳施药量为120 g·m~(-2),其茄病镰刀菌消毒3 d菌量大幅下降,9 d时降为0,种植黄瓜苗后菌量恢复缓慢,对黄瓜根腐病防治效果为66.56%~74.41%,植株长势良好。
译  名：
Studies of Soil Improvement Effect of Calcium Cyanamide and Its Control Efficiency on Soil-borne Diseases of Vegetable Crops
作  者：
BEN Hai-yan;SHI Yan-xia;XIE Xue-wen;CHAI A-li;LI Bao-ju;Gardening Branch,Heilongjiang Academy of Agricultural Sciences;Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences;
关键词：
cucumber;;calcium cyanamide;;Fusarium solani;;control efficiency;;real-time PCR;;dynamic monitoring
摘  要：
This paper reported the effects of calcium cyanamide(CaCN_2) on control of cucumber root rot caused by Fusarium solani. Through the examination of disease control effectiveness,plant growth and other indices,we evaluated the antimicrobial effects of CaCN_2 on F. solani in soil at different doses and different treatment periods by real-time PCR. The minimal detectable concentration of 1 × 101 dRn · g~(-1) soil was established to monitor the population of F. solani. The results showed that CaCN_2 reached its best inhibitory effects on F. solani at the dose of 120 g·m~(-2). In disinfection period,the population of pathogen greatly reduced within 3 days,and little pathogen could be detectable at the 9th day. In plant growing period,the population of this pathogen recovered slowly,and the control efficiency could reach 66.56%–74.41%.

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