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Position: Home > Articles > Isolation and Identification of Newcastle Disease Virus from Egret Progress in Veterinary Medicine 2012,33 (7) 119-121

白鹭源新城疫病毒的分离与鉴定

作  者:
陈安莉;谢芝勋;刘加波;庞耀珊;邓显文;谢志勤;谢丽基;范睛
单  位:
广西大学动物科学技术学院;广西兽医研究所广西畜禽疫苗新技术重点实验室
关键词:
白鹭;新城疫病毒;分离;鉴定
摘  要:
从1只患病的白鹭的咽喉、泄殖腔棉拭子中分离到1株病毒,经血凝、血凝抑制试验和RT-PCR法鉴定为新城疫病毒。根据该毒株对鸡胚平均致死时间、鸡胚半数致死量、鸡胚半数感染量的测定和新城疫强弱毒鉴别的RT-PCR检测,表明该分离株为新城疫强毒株。
译  名:
Isolation and Identification of Newcastle Disease Virus from Egret
作  者:
CHEN An-li1,XIE Zhi-xun2,LIU Jia-bo2,PANG Yao-shan2,DENG Xian-wen2, XIE Zhi-qin2,XIE Li-ji2,FAN Qing2(1.College of Veterinary Medicine,Guangxi University,Nanning,Guangxi,530004,China;2.Guangxi Veterinary Research Institute,Guangxi Key Laboratory of Animal Vaccines and New Technology,Nanning,Guangxi,530001,China)
关键词:
Egret;Newcastle disease virus;isolation;identification
摘  要:
A strain of virus was isolated from injured a Egret with throat and cloacae swabs to be Newcastle disease virus by hemagglutination test and hemagglutination inhibition test and PCR detection.The isolated virus was proved to be a strain of virulent virus by detecting its mean death time to chicken embryo,ELD50 to SPF chicken embryo,EID50 to SPF chicken embryo,and RT-PCR detection.

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