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Position: Home > Articles > Sequence Analysis on ITS1 in Scapharca subcrenata,Tegillarca granosa and Scapharca broughtonii Biotechnology Bulletin 2011,0 (7) 117-120

毛蚶、泥蚶和魁蚶ITS1核苷酸序列分析

作  者:
孙桂金;潘杰;刘可春;王雪;彭维兵;何秋霞;王思锋
单  位:
山东师范大学生命科学学院;山东省科学院生物研究所
关键词:
泥蚶;毛蚶;魁蚶;ITS1;序列分析
摘  要:
对毛蚶、泥蚶和魁蚶3种贝类的ITS1序列进行PCR扩增、测序及分析,并用M ega3.1软件对其进行系统进化分析。毛蚶、泥蚶和魁蚶的ITS1序列长度分别为424、456和431 bp,3种蚶的479个序列位点中,共有330个保守位点,98个变异位点,94个单突变位点。系统进化分析表明,毛蚶和泥蚶先聚为一支,而后与魁蚶聚为一支。ITS1分析结果显示毛蚶和泥蚶种间的遗传关系较近。
译  名:
Sequence Analysis on ITS1 in Scapharca subcrenata,Tegillarca granosa and Scapharca broughtonii
作  者:
Sun Guijin1,2 Pan Jie2 Liu Kechun1 Wang Xue1 Peng Weibing1 He Qiuxia1 Wang Sifeng1(1Biology Institute of Shandong Academy of Sciences,Jinan 250014;2College of Life Sciences,Shandong Normal University,Jinan 250014)
关键词:
Scapharca subcrenata Tegillarca granosa Scapharca broughtonii ITS1 Sequence analysis
摘  要:
Scapharca subcrenata,Tegillarca granosa and Scapharca broughtonii ITS1 sequences were amplified,sequenced and analysed.With the software of Mega3.1,we analysed the phylogenetic development.The results showed that the size of Scapharca subcrenata,Tegillarca granosa and Scapharca broughtonii ITS1 sequences were 424,456 and 431 bp,respectively.There are 479 sequence loci which contain 330 conserved loci,98 variable loci and 94 singleton loci in this three species.The result of phylogenetic development showed that Scapharca subcrenatas and Tegillarca granosa were classified in one branch of phylogenetic tree,then were classified with Scapharca broughtonii in another branch.The analytical result of ITS1 sequences indicate that the genetic relation between Scapharca subcrenata and Tegillarca granosa were close.

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