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Position: Home > Articles > Abundance of resistin mRNA of calf adipocyte in different differentiation stage Chinese Journal of Veterinary Science 2008,28 (1) 84-86

不同分化时期牛脂肪细胞抵抗素mRNA丰度变化

作  者:
宋文华;孙国权;张才;刘国文;车英玉;夏成;王哲;王贵
单  位:
吉林农业大学动物科技学院;吉林大学畜牧兽医学院
关键词:
脂肪细胞;抵抗素;mRNA丰度
摘  要:
选取犊牛大网膜脂肪组织,进行牛前脂肪细胞的单层贴壁培养,在细胞接种4、6、8、10、12、14、16d分别提取细胞总RNA,进行RT-PCR反应,以β-actin为定量内参照,对抵抗素mRNA的丰度进行定量分析,观察在犊牛前脂肪细胞向脂肪细胞分化过程中抵抗素mRNA的表达规律。结果表明:在犊牛前脂肪细胞分化4~12d,抵抗素mRNA丰度逐渐降低,而分化成熟后14~16d有所回升。
译  名:
Abundance of resistin mRNA of calf adipocyte in different differentiation stage
作  者:
SONG Wen-hua1,2,SUN Guo-quan1,3,ZHANG Cai1,LIU Guo-wen1,CHE Ying-yu1,4,XIA Cheng1,WANG Zhe1,WANG Gui4(1.College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,China;2.Songyuan College of Vocabulary and Technology,Songyuan,Jilin 138000,China;3.Inner Mongolia National University,Tongliao,Inner Mongolia 028000,China;4.College of Animal Science and Veterinary Medicine,Jilin Agriculture University,Changchun 130118,China)
关键词:
adipocyte;resistin;mRNA abundance
摘  要:
Preadipocytes from epiploon of calf were cultured with monolayer adherent method,and total RNA of adipocytes was extracted at 4,6,8,10,12,14 and 16 d,respectively.Abundance of resistin mRNA was analysed with intra-reference β-actin by relatively quantitative RT-PCR to observe the expression of resistin mRNA during differentiation from preadipocyte to adipocyte.The result indicated that the abundance of resistin mRNA was down-regulated at 4-12 d of starting differentiation from preadipocyte to adipocyte,but up-regulated slightly 14-16 d after maturation.

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