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Position: Home > Articles > Tissue Culture and Rapid Propagation of Callistemon rigidus Journal of Sichuan Agricultural University 2003,21 (4) 359-360

红千层的组织培养与快速繁殖

作  者:
龚伟;宫渊波;胡庭兴;张健;辜云杰;麻泽龙
单  位:
四川农业大学林学园艺学院
关键词:
红千层;组织培养;快速繁殖;材料保存
摘  要:
以红千层带腋芽茎段为外植体,进行快速繁殖技术研究,其启动率30d可达86 9%,繁殖系数40d可达30~50倍,生根率30d可达95 3%。启动培养基为MS+BA2 0mg/L+NAA0 01mg/L,继代增殖以MS+BA1 0mg/L+NAA0 1mg/L最佳,生根培养以1/2MS无激素效果最好。丛生苗在5℃时可保存160d。
译  名:
Tissue Culture and Rapid Propagation of Callistemon rigidus
作  者:
GONG Wei, GONG Yuan-bo, HU Ting-xing, ZHANG Jian, GU Yun-jie, MA Ze-long (College of Forestry and Horticulture, Sichuan Agricultural University, Yaan 625014, Sichuan, China)
关键词:
Callistemon rigidus; tissue culture; rapid propagation; material preservation
摘  要:
The micropropagation technique of Callistemon rigidus is developed by using section of stem with buds for explants in this study. The induction rate is 86.9% in 30 days; the multiplication coefficient is 30~50 times in 40 days; the rooting rate is 95.3% in 30 days. The initiation medium is MS+BA 2.0?mg/L+NAA 0.01?mg/L; the best subculture medium is MS+BA 1.0?mg/L+NAA 0.1?mg/L; the ideal rooting medium is 1/2 MS without phytohormones. The shoots can be preserved for 160 days at 5?℃.

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