Determination of Albendazole and Its Main Metabolites Residues in Cattle and Ovine Tissues by HPLC-FLD

WU Ning-peng;PENG Li;MENG Lei;CHEN Yan-long;MA Hao-xuan;Henan Institute of Veterinary Drug and Feeds Control;Zhengzhou University;Henan Province Zhengzhou No.7 Middle School;

cattle and ovine tissues;;albendazole and its main metabolites;;HPLC-FLD

A method based on high performance liquid chromatography with fluorescence detector( HPLC-FLD)has been developed for the determination of albendazole and its main metabolites residues in cattle and ovine tissues. The analytes were extracted with ethyl acetate,and cleaned by Alumina-A and MCX solid phase extraction cartridges. Quantification of the analytes were achieved by HPLC-FLD using external standard method. The calibration curves of the drugs were linear in the concentration ranges of 5 ~ 2000 μg/L with correlation coefficients more than 0. 999. Detection limits and quantification limits of the method for the analytes were 0. 025 mg/kg and 0. 05 mg/kg in cattle and ovine tissues. The average recoveries ranged from 60. 6% to119. 1% at the spiked level of 0. 05 ~ 10 mg/kg in tissues. The relative standard deviation was in the range of1. 7% ~ 19. 1%. With the advantages of good anti-interference ability and sensitivity,the method adapts to the determination of albendazole and its main metabolites residues in cattle and ovine tissues.