Position: Home > Articles > Construction of Maize Universal Expression Vector PGM-35Sbar and Maize Transformation
Journal of Anhui Agricultural Sciences
2012,40
(25)
12367-12370
PGM-35Sbar玉米通用表达载体的构建及玉米转化研究
作 者：
王霄汉;罗昌;程曦;张秀海;黄丛林;吴忠义
单 位：
首都师范大学生命科学学院;北京市农林科学院技术研究中心
关键词：
玉米通用表达载体;花粉管通道法;除草剂抗性;转基因玉米
摘 要：
[目的]构建玉米通用表达载体,以期为利用转基因手段提高玉米对非生物胁迫的耐受性奠定基础。[方法]通过对现有的pGreen0229植物表达载体进行改造,构建含有CaMv35S启动子驱动的抗草胺膦筛选基因bar、可用于连接目的基因的玉米表达载体PGM-35Sbar,并通过花粉管通道法转化吉444玉米自交系。[结果]PGM-35Sbar玉米通用载体构建成功,转化玉米植株后,得到抗性植株14棵,经PCR检测其中有12棵为阳性植株。[结论]该研究为快速构建含有特定目的基因的玉米表达载体奠定了基础。
译 名：
Construction of Maize Universal Expression Vector PGM-35Sbar and Maize Transformation
作 者：
WANG Xiao-han et al(College of Life Science,Capital Normal University,Beijing 100048)
关键词：
Maize universal expression vector;Pollen-tube pathway;Herbicide resistance;Transgenic maize
摘 要：
[Objective] The paper was to construct maize universal expression vector,in order to provide basis for using transgenic methods to improve abiotic stress tolerance of maize.[Method] Based on the transformation of existing pGreen0229 plant expression vector,phosphinothricin-resistant selectable marker-bar gene driving by CaMv35S promoter was constructed,which could be used to connect target gene of maize expression vector PGM-35Sbar,and transform Ji444 maize inbred lines by pollen tube pathway.[Result] The universal expression vector for PGM-35Sbar maize had been successfully constructed.When the maize plants were transformed,14 resistant plants were obtained,and 12 plants were identified to be positive plants by PCR detection.[Conclusion] The study provided basis for rapid construction of maize expression vector containing specific target gene.
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